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RNF40 Antibody (1C1)

Novus Biologicals, part of Bio-Techne | Catalog # H00009810-M09

Novus Biologicals, part of Bio-Techne
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H00009810-M09

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 1C1

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

RNF40 (NP_055586, 102 a.a. ~ 200 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. DETVEALLRCHESQGELSSAPEAPGTQEGPTCDGTPLPEPGTSELRDPLLMQLRPPLSEPALAFVVALGASSSEEVELELQGRMEFSKAAVSRVVEASD

Specificity

RNF40 - ring finger protein 40 (1C1)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for RNF40 Antibody (1C1)

Western Blot: RNF40 Antibody (1C1) [H00009810-M09]

Western Blot: RNF40 Antibody (1C1) [H00009810-M09]

Western Blot: RNF40 Antibody (1C1) [H00009810-M09] - Analysis of RNF40 expression in transfected 293T cell line by RNF40 monoclonal antibody (M09), clone 1C1. Lane 1: RNF40 transfected lysatE (113.7 KDa). Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: RNF40 Antibody (1C1) [H00009810-M09]

Immunocytochemistry/ Immunofluorescence: RNF40 Antibody (1C1) [H00009810-M09]

Immunocytochemistry/Immunofluorescence: RNF40 Antibody (1C1) [H00009810-M09] - Analysis of monoclonal antibody to RNF40 on HeLa cell. Antibody concentration 10 ug/ml
ELISA: RNF40 Antibody (1C1) [H00009810-M09]

ELISA: RNF40 Antibody (1C1) [H00009810-M09]

ELISA: RNF40 Antibody (1C1) [H00009810-M09] - Detection limit for recombinant GST tagged RNF40 is approximately 1ng/ml as a capture antibody.

Applications for RNF40 Antibody (1C1)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against transfected lysate and recombinant protein for western blot. It has also been used for ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: RNF40

The protein encoded by this gene contains a RING finger, a motif known to be involved in protein-protein and protein-DNA interactions. This protein was reported to interact with the tumor suppressor protein RB1. Studies of the rat counterpart suggested that this protein may function as an E3 ubiquitin-protein ligase, and facilitate the ubiquitination and degradation of syntaxin 1, which is an essential component of the neurotransmitter release machinery. [provided by RefSeq]

Alternate Names

95 kDa retinoblastoma-associated protein, BRE1-B, BRE1BMGC13051, EC 6.3.2,95 kDa retinoblastoma protein binding protein, EC 6.3.2.-, KIAA0661BRE1 E3 ubiquitin ligase homolog B, Rb-associated protein, RBP95E3 ubiquitin-protein ligase BRE1B, ring finger protein 40DKFZp686K191, STARING

Entrez Gene IDs

9810 (Human)

Gene Symbol

RNF40

Additional RNF40 Products

Product Documents for RNF40 Antibody (1C1)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for RNF40 Antibody (1C1)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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