RNF40 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-2247

Novus Biologicals, part of Bio-Techne
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NB100-2247
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Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.2 mg/ml

View all RNF40 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 540 and 590 of human Ring Finger 40 using the numbering given in entry NP_055586.1 (GeneID 9810).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RNF40 Antibody

Western Blot: RNF40 Antibody [NB100-2247]

Western Blot: RNF40 Antibody [NB100-2247]

Western Blot: RNF40 Antibody [NB100-2247] - Detection of Human RNF40 on HeLa whole cell lysate using NB100-2247. RNF40 was also immunoprecipitated using rabbit anti-RNF40 antibodies NB100-2246 and NB100-2248.
Immunohistochemistry-Paraffin: RNF40 Antibody [NB100-2247]

Immunohistochemistry-Paraffin: RNF40 Antibody [NB100-2247]

Immunohistochemistry-Paraffin: RNF40 Antibody [NB100-2247] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti- RNF40 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB

Applications for RNF40 Antibody

Application
Recommended Usage

Immunohistochemistry

1:100-1:500

Immunohistochemistry-Paraffin

1:100-1:500

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: RNF40

The conjugation of ubiquitin to proteins is an important means to regulate protein activity for many cellular processes. Targeting of proteins by ubiquitination occurs in three main steps that involve the activation of ubiquitin by E1 enzymes, the carrying of ubiquitin to the target protein by E2 enzymes and the ligation of ubiquitin to the target protein by E3 enzymes. Ring finger protein 40 (RNF40) is a member of the RING type E3 ubiquitin ligases. RNF40 forms a complex with RNF20 to carry out H2B monoubiquitination in association with hPAF and the UbcH6 ubiquitin E2-conjugating enzyme. H2B monoubiquitination by RN40/RNF20 has been shown to be associated with increased H3 methylation and gene transcription. Alternate names for RNF40 include E3 ubiquitin-protein ligase BRE1B, BRE1-B, 95kDa retinoblastoma-associated protein, RBP95, STARING, KIAA0661, MGC13051, and DKFZp686K191.

Alternate Names

95 kDa retinoblastoma-associated protein, BRE1-B, BRE1BMGC13051, EC 6.3.2,95 kDa retinoblastoma protein binding protein, EC 6.3.2.-, KIAA0661BRE1 E3 ubiquitin ligase homolog B, Rb-associated protein, RBP95E3 ubiquitin-protein ligase BRE1B, ring finger protein 40DKFZp686K191, STARING

Entrez Gene IDs

9810 (Human)

Gene Symbol

RNF40

UniProt

NP_055586.1 (Human)

Additional RNF40 Products

Product Documents for RNF40 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for RNF40 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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