RNF44 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-82930
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: MRPWALAVTRWPPSAPVGQRRFSAGPGSTPGQLWGSPGLEGPLASPPARDERLPSQQPPSRPPHLPVEERRASAPAGGSPRMLHPATQ
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for RNF44 Antibody
Western Blot: RNF44 Antibody [NBP1-82930]
Western Blot: RNF44 Antibody [NBP1-82930] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG spImmunocytochemistry/ Immunofluorescence: RNF44 Antibody [NBP1-82930]
Immunocytochemistry/Immunofluorescence: RNF44 Antibody [NBP1-82930] - Staining of human cell line A-431 shows positivity in nucleus but not nucleoli.Immunohistochemistry-Paraffin: RNF44 Antibody [NBP1-82930]
Immunohistochemistry-Paraffin: RNF44 Antibody [NBP1-82930] - Staining of human testis shows moderate nuclear positivity in cells in seminiferus ducts.Applications for RNF44 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:20 - 1:50
Immunohistochemistry-Paraffin
1:20 - 1:50
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: RNF44
Alternate Names
KIAA1100, ring finger protein 44
Gene Symbol
RNF44
Additional RNF44 Products
Product Documents for RNF44 Antibody
Product Specific Notices for RNF44 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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