RNF70 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-87399

Novus Biologicals, part of Bio-Techne
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NBP1-87399
NBP1-87399-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all RNF70 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: LPARPSRAPVSICGGGENTSKSAEEPVVRPKIRNLASPNCVKPKIFFDTDDDDDMPHSTSRWRDTANDNEGHSDGLARRGRGESSSGYPEPKYPEDKREARSDQVKP

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RNF70 Antibody

Western Blot: RNF70 Antibody [NBP1-87399]

Western Blot: RNF70 Antibody [NBP1-87399]

Western Blot: RNF70 Antibody [NBP1-87399] - Analysis in human cell line RT-4.
Immunocytochemistry/ Immunofluorescence: RNF70 Antibody [NBP1-87399]

Immunocytochemistry/ Immunofluorescence: RNF70 Antibody [NBP1-87399]

Immunocytochemistry/Immunofluorescence: RNF70 Antibody [NBP1-87399] - Staining of human cell line U-2 OS shows localization to nucleus and nucleoli.
Immunohistochemistry-Paraffin: RNF70 Antibody [NBP1-87399]

Immunohistochemistry-Paraffin: RNF70 Antibody [NBP1-87399]

Immunohistochemistry-Paraffin: RNF70 Antibody [NBP1-87399] - Staining in human cerebral cortex and liver tissues using anti-PJA1 antibody. Corresponding PJA1 RNA-seq data are presented for the same tissues.
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Applications for RNF70 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RNF70

RNF70 has E2-dependent E3 ubiquitin-protein ligase activity. Ubiquitinates MAGED1 antigen leading to its subsequentdegradation by proteosome . May be involved in protein sorting

Alternate Names

EC 6.3.2, EC 6.3.2.-, FLJ11830, praja 1, praja ring finger 1, PRAJA1, RING finger protein 70, RNF70E3 ubiquitin-protein ligase Praja-1

Gene Symbol

PJA1

Additional RNF70 Products

Product Documents for RNF70 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for RNF70 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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