RNMT Antibody (3H3-1D12)

Novus Biologicals, part of Bio-Techne | Catalog # H00008731-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 3H3-1D12

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all RNMT Antibodies »

Product Specifications

Immunogen

RNMT (AAH36798, 1 a.a. ~ 476 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MANSAKAEEYEKMSLEQAKASVNSETESSFNINENTTASGTGLSEKTSVCRQVDIARKRKEFEDDLVKESSSCGKDTPSKKRKLDPEIVPEEKDCGDAEGNSKKRKRETEDVPKDKSSTGDGTQNKRKIALEDVPEKQKNLEEGHSSTVAAHYNELQEVGLEKRSQSRIFYLRNFNNWMKSVLIGEFLEKVRQKKKRDITVLDLGCGKGGDLLKWKKGRINKLVCTDIADVSVKQCQQRYEDMKNRRDSEYIFSAEFITADSSKELLIDKFRDPQMCFDICSCQFVCHYSFESYEQADMMLRNACERLSPGGYFIGTTPNSFELIRRLEASETESFGNEIYTVKFQKKGDYPLFGCKYDFNLEGVVDVPEFLVYFPLLNEMAKKYNMKLVYKKTFLEFYEEKIKNNENKMLLKRMQALEPYPANESSKLVSEKVDDYEHAAKYMKNSQVRLPLGTLSKSEWEATSIYLVFAFEKQQ

Localization

Nucleus

Specificity

RNMT - RNA (guanine-7-) methyltransferase

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for RNMT Antibody (3H3-1D12)

Western Blot: RNMT Antibody (3H3-1D12) [H00008731-M01]

Western Blot: RNMT Antibody (3H3-1D12) [H00008731-M01]

Western Blot: RNMT Antibody (3H3-1D12) [H00008731-M01] - RNMT monoclonal antibody (M01), clone 3H3-1D12 Analysis of RNMT expression in HeLa.
Immunocytochemistry/ Immunofluorescence: RNMT Antibody (3H3-1D12) [H00008731-M01]

Immunocytochemistry/ Immunofluorescence: RNMT Antibody (3H3-1D12) [H00008731-M01]

Immunocytochemistry/Immunofluorescence: RNMT Antibody (3H3-1D12) [H00008731-M01] - Analysis of monoclonal antibody to RNMT on HeLa cell. Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: RNMT Antibody (3H3-1D12) [H00008731-M01]

Immunohistochemistry-Paraffin: RNMT Antibody (3H3-1D12) [H00008731-M01]

Immunohistochemistry-Paraffin: RNMT Antibody (3H3-1D12) [H00008731-M01] - Analysis of monoclonal antibody to RNMT on formalin-fixed paraffin-embedded human squamous cell carcinoma tissue. Antibody concentration 3 ug/ml.
View 1 more images

Applications for RNMT Antibody (3H3-1D12)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF, IHC-P and ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: RNMT

mRNA cap methyltransferase (HCM) catalyzes the N7 methylation of the 5' cap formed by mRNA capping enzyme (HCE) to form a terminal 7-methylguanosine. This is subsequently recognised by cap binding proteins that facilitate key events in gene expression.

Alternate Names

DKFZp686H1252, EC 2.1.1.56, hcm1p, hCMT1, hCMT1c, hMet, KIAA0398, mRNA (guanine-7-)methyltransferase, mRNA (guanine-N(7)-)-methyltransferase, mRNA cap guanine-N7 methyltransferase, mRNA cap methyltransferase, RG7MT1MET, RNA (guanine-7-) methyltransferase

Entrez Gene IDs

8731 (Human)

Gene Symbol

RNMT

OMIM

603514 (Human)

UniProt

AAH36798 (Human)

Additional RNMT Products

Product Documents for RNMT Antibody (3H3-1D12)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for RNMT Antibody (3H3-1D12)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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