RNPEPL1 Antibody (4A9)

Western Blot: RNPEPL1 Antibody (4A9) [NBP2-59371]

Western Blot: RNPEPL1 Antibody (4A9) [NBP2-59371] - Western Blot analysis of N-terminal Arginine-BSA showing detection of 67 kDa N-terminal Arginylation protein using Mouse Anti-N-terminal Arginylation Monoclonal Antibody, Clone 4A9 (SMC-263). Lane 1: Molecular Weight Ladder (MW). Lane 2: BSA. Lane 3: RDHKH-BSA. Lane 4: REHKH-BSA. Lane 5: HKH-BSA. Lane 6: HKERD-BSA. Lane 7: HKRRE-BSA. Load: 0.5 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-N-terminal Arginylation Monoclonal Antibody (SMC-263) at 1:1000 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution (Super Signal West Pico) for 5 min in RT. Predicted/Observed Size: 67 kDa. Other Band(s): 250kDa, 150kDa, 75kDa REHKH-BSA.

Flow Cytometry: RNPEPL1 Antibody (4A9) [NBP2-59371]

Flow Cytometry: RNPEPL1 Antibody (4A9) [NBP2-59371] - Flow Cytometry analysis using Mouse Anti-RNPEPL1 Monoclonal Antibody, Clone 4A9 (NBP2-59371). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 90% Methanol. Primary Antibody: Mouse Anti-RNPEPL1 Monoclonal Antibody (NBP2-59371) at 1:50 for 30 min on ice. Secondary Antibody: Goat Anti-Mouse: PE at 1:100 for 20 min at RT. Isotype Control: Non Specific IgG. Heat stressed cells were subject to heat shock at 42C for 2 hours.