ROBO1 Antibody (10E2-R)

Western Blot: ROBO1 Antibody (10E2-R) [NBP3-32913]

Western Blot: ROBO1 Antibody (10E2-R) [NBP3-32913] - Western blot analysis of ROBO1 on different lysates with Mouse anti-ROBO1 antibody (NBP3-32913) at 1/1,000 dilution.

Lane 1: MCF-7 cell lysate (10 ug/Lane)
Lane 2: NIH/3T3 cell lysate (10 ug/Lane)
Lane 3: Rat brain tissue lysate (20 ug/Lane)
Lane 4: PC-12 cell lysate (10 ug/Lane)
Lane 5: RAW264.7 cell lysate (10 ug/Lane)

Predicted band size: 181 kDa
Observed band size: 181 kDa

Exposure time: 30 seconds;

6% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32913) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: ROBO1 Antibody (10E2-R) [NBP3-32913]

Immunohistochemistry: ROBO1 Antibody (10E2-R) [NBP3-32913] - Immunohistochemical analysis of paraffin-embedded human breast tissue with Rabbit anti-ROBO1 antibody (NBP3-32913) at 1/600 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32913) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.