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ROBO2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-23374

Novus Biologicals, part of Bio-Techne
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NBP3-23374

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Chicken

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Summary for ROBO2 Antibody

Immunogen

Robo2 synthetic peptide (coupled to carrier protein) corresponds to amino acids in the C-terminal region of human Robo2. This sequence has significant homology to the same region in rat and mouse Robo2, and has low homology to other Robo family members. [UniProt# Q9HCK4]

Specificity

The antibody detects a 175 kDa band corresponding to Robo2 in Western blots of mouse C2C12 and SYF cells.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

175 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Stable for at least 1 year at -20°C.

Scientific Data Images for ROBO2 Antibody

ROBO2 Antibody

Western Blot: ROBO2 Antibody [NBP3-23374] -

Western Blot: ROBO2 Antibody [NBP3-23374] - Western blot analysis of C2C12 and SYF mouse cell lines. The blots were probed with anti-Robo2 (C-terminal region) in the absence (-) or presence (+) of ROBO2 (C-terminal region) blocking peptide.

Applications for ROBO2 Antibody

Application
Recommended Usage

Western Blot

1:500
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1 mg/ml BSA and 50% glycerol

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C.

Background: ROBO2

Robo2 belongs to the ROBO family, part of the immunoglobulin superfamily proteins that are highly conserved from fly to human. The encoded protein is a receptor for SLIT2, molecules known to function in axon guidance and cell migration. Defects in this gene are the cause of vesicoureteral reflux type 2. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq]

Long Name

Roundabout Homolog 2

Alternate Names

SAX3

Gene Symbol

ROBO2

Additional ROBO2 Products

Product Documents for ROBO2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for ROBO2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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