ROBO3 Antibody (2H1)
Novus Biologicals, part of Bio-Techne | Catalog # H00064221-M02
Key Product Details
Species Reactivity
Human
Applications
ELISA, Sandwich ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 2H1
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
ROBO3 (AAH08623, 1 a.a. ~ 147 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MTPPLQGPRARFRKKPKALPYRRENSPGDLPPPPLPPPEEEASWALELRAAGSMSSLERERSGERKAVQAVPLAAQRVLHPDEEAWLPYSRPSFLSRGQGTSTCSTAGSNSSRGSSSSRGSRGPGRSRSQSRSQSQRPGQKRREEPR
Specificity
Reacts with roundabout, axon guidance receptor, homolog 3 (Drosophila).
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Scientific Data Images for ROBO3 Antibody (2H1)
![Western Blot: ROBO3 Antibody (2H1) [H00064221-M02]](https://resources.bio-techne.com/images/products/ROBO3-Antibody-2H1-Western-Blot-H00064221-M02-img0002.jpg "Western Blot: ROBO3 Antibody (2H1) [H00064221-M02]")
Western Blot: ROBO3 Antibody (2H1) [H00064221-M02]
Western Blot: ROBO3 Antibody (2H1) [H00064221-M02] - Analysis of ROBO3 expression in transfected 293T cell line by ROBO3 monoclonal antibody (M02), clone 2H1.Lane 1: ROBO3 transfected lysate (Predicted MW: 16.1 KDa).Lane 2: Non-transfected lysate.
Sandwich ELISA: ROBO3 Antibody (2H1) [H00064221-M02] - Detection limit for recombinant GST tagged ROBO3 is 0.1 ng/ml as a capture antibody.
Applications for ROBO3 Antibody (2H1)
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Sandwich ELISA
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This antibody is reactive against recombinant protein in western blot and ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: ROBO3
Long Name
Roundabout Homolog 3
Alternate Names
RIG1
Entrez Gene IDs
64221 (Human)
Gene Symbol
ROBO3
UniProt
Additional ROBO3 Products
Product Documents for ROBO3 Antibody (2H1)
Product Specific Notices for ROBO3 Antibody (2H1)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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