ROCK1 Antibody (464)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-42918

Novus Biologicals, part of Bio-Techne
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NBP2-42918
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Key Product Details

Validated by

Knockout/Knockdown, Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 464

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all ROCK1 Antibodies »

Product Summary for ROCK1 Antibody (464)

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human ROCK1. The exact sequence is proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for ROCK1 Antibody (464)

Western Blot: ROCK1 Antibody (464) [NBP2-42918]

Western Blot: ROCK1 Antibody (464) [NBP2-42918]

Western Blot: ROCK1 Antibody (464) [NBP2-42918] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ROCK1 antibody [GT464].
Western Blot: ROCK1 Antibody (464) [NBP2-42918]

Western Blot: ROCK1 Antibody (464) [NBP2-42918]

Western Blot: ROCK1 Antibody (464) [NBP2-42918] - Analysis of A. 30 ug 293T whole cell lysate/extract B. 30 ug HeLa whole cell lysate/extract C. 30 ug HepG2 whole cell lysate/extract 5 % SDS-PAGE ROCK1 antibody [464] dilution: 1:1000
Western Blot: ROCK1 Antibody (464) [NBP2-42918]

Western Blot: ROCK1 Antibody (464) [NBP2-42918]

Western Blot: ROCK1 Antibody (464) [NBP2-42918] - Analysis of A. 30 ug Neuro2A whole cell lysate/extract B. 30 ug GL261 whole cell lysate/extract C. 30 ug NIH-3T3 whole cell lysate/extract D. 30 ug BCL-1 whole cell lysate/extract E. 30 ug Raw264.7 whole cell lysate/extract F. 30 ug C2C12 whole cell lysate/extract 5 % SDS-PAGE ROCK1 antibody [464] dilution: 1:1000
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Applications for ROCK1 Antibody (464)

Application
Recommended Usage

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: ROCK1

Rho-associated kinase I (ROCK1, ROK beta, p160ROCK) is a serine-threonine protein kinase and an effector of the small GTPase Rho. With N-terminus protein kinase domain and C-terminus Rho-binding domain/pleckstrin homology domain, ROCK1 can be activated by either RhoA or RhoB. In Rho specific ROCK1 activation, a Rho protein binds to the Rho-binding domain and induces a conformational change which opens the kinase domain for the phosphorylation of downstream effectors (1). Activated ROCK1 phosphorylates various signaling proteins, such as myosin light chain phosphatase, LIM kinases, and ezrin-radixin-moesin proteins. Caspase-3 also activates ROCK1; Caspase-3 cleaves ROCK1 at DETD1113/G sequence and removes its inhibitory c-terminal domain. This activation, independent of Rho activity, leads to apoptotic membrane blebbing (2). ROCK1 is involved in regulating actin cytoskeleton assembly, cell migration, centromere positing, smooth muscle contraction, and neurite outgrowth. Its involvement in tumor invasion, hypertension, and bronchial asthma makes ROCK1 an ideal target for drug development (3).

Long Name

Rho-associated, Coiled-Coil Containing Protein Kinase 1

Alternate Names

p160ROCK, ROK beta

Gene Symbol

ROCK1

Additional ROCK1 Products

Product Documents for ROCK1 Antibody (464)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for ROCK1 Antibody (464)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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