Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human

Predicted:

Bovine (100%), Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunoprecipitation, Western Blot

Cited:

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all ROCK2 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residues 1320 and the C-terminus (residues 1388) of human ROCK2 using the number given in entry NP_004841.1 (GeneID 9475)

Specificity

NB100-593 is specific for human Rho Kinase alpha protein.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ROCK2 Antibody

Western Blot: ROCK2 Antibody [NB100-593]

Western Blot: ROCK2 Antibody [NB100-593]

Western Blot: ROCK2 Antibody [NB100-593] - Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ROCK2 antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Western Blot: ROCK2 Antibody [NB100-593]

Western Blot: ROCK2 Antibody [NB100-593]

Western Blot: ROCK2 Antibody [NB100-593] - Detection of Human Rho Kinase Alpha on HeLa whole cell lysate using NB100-593. NB100-593 and NB100-592 were used at 5 ug/mg lysate to immunoprecipitate Rho Kinase Alpha.

Applications for ROCK2 Antibody

Application
Recommended Usage

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:10000-1:25000
Application Notes
Optimal working dilutions should be determined experimentally by the investigator. Prepare working dilution immediately before use. Suggested starting dilutions are as follows. Western Blot: 1:10,000 - 1:25,000 Immunoprecipitation: 2 to 5 ug/mg of lysate Histo/Cytochemistry: not tested *In some cases, the antibody may be diluted further than indicated.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: ROCK2

The small GTPase Rho is implicated in cytoskeletal rearrangements including stress fiber and focal adhesion formation and in the transcriptional activation of c-fos serum response element. In vitro, Rho-kinase, which is activated by Rho, phosphorylates not only myosin light chain (MLC) (thereby activating myosin ATPase) but also myosin phosphatase, thus inactivating myosin phosphatase. Rho-kinase is involved in the formation of stress fibers and focal adhesions in fibroblasts.

Long Name

Rho-associated, Coiled-coil Containing Protein Kinase 2

Alternate Names

ROCK-II, ROKalpha

Entrez Gene IDs

9475 (Human)

Gene Symbol

ROCK2

UniProt

O75116

Additional ROCK2 Products

Product Documents for ROCK2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for ROCK2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.