RPA14 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-32023

Novus Biologicals, part of Bio-Techne
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NBP1-32023
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Rhesus Macaque (94%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all RPA14 Antibodies »

Product Summary for RPA14 Antibody

Immunogen

Full length human RPA14 Recombinant protein.

Reactivity Notes

Bovine (85%).

Localization

Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

14 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for RPA14 Antibody

Immunohistochemistry-Paraffin: RPA14 Antibody [NBP1-32023]

Immunohistochemistry-Paraffin: RPA14 Antibody [NBP1-32023]

Immunohistochemistry-Paraffin: RPA14 Antibody [NBP1-32023] - Paraffin-embedded lung CA patient tumor, using antibody at 1:100 dilution.
RPA14 Antibody

Western Blot: RPA14 Antibody [NBP1-32023] -

Western Blot: RPA14 Antibody [NBP1-32023] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with RPA14 antibody (NBP1-32023) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for RPA14 Antibody

Application
Recommended Usage

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: RPA14

Replication protein A (RPA) is a single-stranded DNA binding protein. Human RPA is a heterotrimeric protein containing subunits of 70, 32 and 14 kDa. This protein complex is highly conserved in eukaryotes and is essential in DNA replication, homologous recombination and nucleotide excision repair. RPA has been shown to be a target for DNA-dependent protein kinase (DNA-PK) ataxia telangiectasia-mutated gene (ATM) and a cyclin dependent protein kinase.

Alternate Names

REPA3Replication factor A protein 3, replication protein A 14 kDa subunit, replication protein A3 (14kD), replication protein A3, 14kDa, RF-A protein 3, RP-A p14, RPA14

Gene Symbol

RPA3

Additional RPA14 Products

Product Documents for RPA14 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for RPA14 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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