RPA2 Antibody (12F3.3)

Novus Biologicals, part of Bio-Techne | Catalog # NB100-158

Novus Biologicals, part of Bio-Techne
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NB100-158
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Chromatin Immunoprecipitation (ChIP), Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Cited:

Chemotaxis

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 12F3.3

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all RPA2 Antibodies »

Product Specifications

Immunogen

Full-length human RPA-32 expressed in E. coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

29 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for RPA2 Antibody (12F3.3)

Western Blot: RPA2 Antibody (12F3.3) [NB100-158]

Western Blot: RPA2 Antibody (12F3.3) [NB100-158]

Western Blot: RPA2 Antibody (12F3.3) [NB100-158] - HeLa (IR treated) whole cell extract. No radiation, 1hr, 2hr, and 4hr exposure corresponding to lanes 1 through 4 respectively.
Immunocytochemistry/ Immunofluorescence: RPA2 Antibody (12F3.3) [NB100-158]

Immunocytochemistry/ Immunofluorescence: RPA2 Antibody (12F3.3) [NB100-158]

Immunocytochemistry/Immunofluorescence: RPA2 Antibody (12F3.3) [NB100-158] - 2mM hydroxyurea treated (right) or untreated (left) HeLa cells were fixed in ice-cold MeOH for 5 min. Green: RPA32 protein stained by RPA32 antibody [12F3.3] Blue: Hoechst 33342 staining.
Western Blot: RPA2 Antibody (12F3.3) [NB100-158]

Western Blot: RPA2 Antibody (12F3.3) [NB100-158]

Western Blot: RPA2 Antibody (12F3.3) [NB100-158] - Various whole cell extracts (30 ug) were separated by 12 % SDS-PAGE, and blotted with RPA32 antibody diluted by 1:500

Applications for RPA2 Antibody (12F3.3)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Western Blot

1:500-1:3000
Application Notes
For IP: Use at an assay dependent dilution. Use in chromatin immunoprecipitation reported in scientific literature (PMID: 31390554).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RPA2

Replication protein A (RPA) is a single-stranded DNA binding protein. Human RPA is a heterotrimeric protein containing subunits of 70, 32, and 14 kDa. This protein complex is highly conserved in eukaryotes and is essential in DNA replication, homologous recombination, and nucleotide excision repair. RPA has been shown to be a target for DNA-dependent protein kinase (DNA-PK), ataxia telangiectasia-mutated gene (ATM), and a cyclin dependent protein kinase. The middle subunit, RPA-32, is phosphorylated during the S-phase of the cell cycle, in response to DNA damage and during apoptotsis of Jurkat T- lymphocytes.

Long Name

Replication Protein A2, 32kDa

Alternate Names

REPA2, RPA32

Entrez Gene IDs

6118 (Human); 19891 (Mouse)

Gene Symbol

RPA2

UniProt

P15927 (Human)

Additional RPA2 Products

Product Documents for RPA2 Antibody (12F3.3)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for RPA2 Antibody (12F3.3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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