RPA2 [p Ser8, p Ser4] Antibody (BL-165-5F1)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-76396
Recombinant Monoclonal Antibody.
Key Product Details
Validated by
Independent Antibodies, Biological Validation
Species Reactivity
Validated:
Human
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # BL-165-5F1
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for RPA2 [p Ser8, p Ser4] Antibody (BL-165-5F1)
Immunogen
Immunogen was a dually phosphorylated synthetic peptide, which represented a portion of human replication protein A2, 32 kDa surrounding phosphorylated serines that corresponded to positions 4 and 8 using the numbering given in entry NP_002937.1 (GeneID 6118).
Modification
p Ser8, p Ser4
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for RPA2 [p Ser8, p Ser4] Antibody (BL-165-5F1)
Western Blot: RPA2 [p Ser8, p Ser4] Antibody (BL-165-5F1) [NBP2-76396] - Detection of human Phospho RPA32 (S4/S8) by WB of HeLa lysate treated with 100 uM EPE (+) or mock treated (-). Antibodies: Rabbit anti-Phospho RPA32 (S4/S8) recombinant monoclonal [BL-165-5F1] (NBP2-76396). Secondary: HRP-conjugated goat anti-rabbit IgG.
Immunocytochemistry/Immunofluorescence: RPA2 [p Ser8, p Ser4] Antibody (BL-165-5F1) [NBP2-76396] - Rabbit anti-Phospho RPA32 (S4/S8) recombinant monoclonal antibody [BL-165-5F1] .Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.
Immunocytochemistry/Immunofluorescence: RPA2 [p Ser8, p Ser4] Antibody (BL-165-5F1) [NBP2-76396] - Detection of Human Phospho RPA32 (S4/S8) in NBF-fixed HeLa cells treated with EPE (right) or mock treated (left) by ICC-IF. Antibody: Rabbit anti-Phospho RPA32 (S4/S8) recombinant monoclonal [BL-165-5F1] (NBP2-76396). Detection: DyLight® 594-conjugated goat anti-rabbit IgG. Counterstain: DAPI (blue).
Applications for RPA2 [p Ser8, p Ser4] Antibody (BL-165-5F1)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:1000 - 1:5000
Immunoprecipitation
6 ul/1.0 mg lysate
Western Blot
1:1000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
Borate Buffered Saline (BBS) pH 8.2
Preservative
0.09% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 2 - 8 C / 1 year from date of receipt
Background: RPA2
Long Name
Replication Protein A2, 32kDa
Alternate Names
REPA2, RPA32
Gene Symbol
RPA2
Additional RPA2 Products
Product Documents for RPA2 [p Ser8, p Ser4] Antibody (BL-165-5F1)
Product Specific Notices for RPA2 [p Ser8, p Ser4] Antibody (BL-165-5F1)
This Product sold under License from Bethyl Laboratories, Inc.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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