RPA2 Antibody (OTI9A1)

Flow Cytometry: RPA2 Antibody (OTI9A1) [NBP2-02182]

Flow Cytometry: RPA2 Antibody (OTI9A1) [NBP2-02182] - Analysis of Jurkat cells, using anti-RPA2 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Western Blot: RPA2 Antibody (OTI9A1) [NBP2-02182]

Western Blot: RPA2 Antibody (OTI9A1) [NBP2-02182] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RPA2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RPA2.

Western Blot: RPA2 Antibody (OTI9A1) [NBP2-02182]

Western Blot: RPA2 Antibody (OTI9A1) [NBP2-02182] - Analysis of extracts (35ug) from 9 different cell lines by usin g anti-RPA2 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Immunohistochemistry-Paraffin: RPA2 Antibody (OTI9A1) [NBP2-02182]

Immunohistochemistry-Paraffin: RPA2 Antibody (OTI9A1) [NBP2-02182] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-RPA2 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: RPA2 Antibody (OTI9A1) [NBP2-02182]

Immunohistochemistry-Paraffin: RPA2 Antibody (OTI9A1) [NBP2-02182] - Staining of paraffin-embedded Human colon tissue using anti-RPA2 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: RPA2 Antibody (OTI9A1) [NBP2-02182]

Immunohistochemistry-Paraffin: RPA2 Antibody (OTI9A1) [NBP2-02182] - Staining of paraffin-embedded Human prostate tissue using anti-RPA2 mouse monoclonal antibody.

Flow Cytometry: RPA2 Antibody (OTI9A1) [NBP2-02182]

Flow Cytometry: RPA2 Antibody (OTI9A1) [NBP2-02182] - Analysis of Hela cells, using anti-RPA2 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Immunoprecipitation: RPA2 Antibody (OTI9A1) [NBP2-02182]

Immunoprecipitation: RPA2 Antibody (OTI9A1) [NBP2-02182] - (Negative control: IP without adding anti-RPA2 antibody.). For each experiment, 500ul of DDK tagged RPA2 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-RPA2 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.

Western Blot: RPA2 Antibody (OTI9A1) [NBP2-02182] -

Western Blot: RPA2 Antibody (OTI9A1) [NBP2-02182] - Ajuba associates with RPA in HTC75 & IMR90 cells. (A) IP-Western probed with a total anti-RPA32 antibody after immunoprecipitation with a commercial anti-Ajuba antibody (com.) or anti-peptide serum from IMR90 or HTC75 extracts as indicated. Left panel: total lysates (input) for each cell line, & the pre-immune serum (PI) used as a control for the immunoprecipitation. (B) Western for RPA32-p-Thr21 in both HTC75 & IMR90Ajuba-depleted cells. A non-specific band is marked by a *. Total RPA32 levels are shown at the bottom. (C) Model for the role of Ajuba in repression of ATR. The “?” indicates that the interaction between Ajuba & RPA could be direct or indirect. See text for details. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/23755068), licensed under a CC-BY license. Not internally tested by Novus Biologicals.