RPA70 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-20206

Novus Biologicals, part of Bio-Techne
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NBP2-20206
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (93%), Sheep (93%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all RPA70 Antibodies »

Product Summary for RPA70 Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human RPA70. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (89%).

Localization

Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

68 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for RPA70 Antibody

Western Blot: RPA70 Antibody [NBP2-20206]

Western Blot: RPA70 Antibody [NBP2-20206]

Western Blot: RPA70 Antibody [NBP2-20206] - Sample (30 ug of whole cell lysate) A: 293T 7. 5% SDS PAGE gel, diluted at 1:5000.
Immunohistochemistry-Paraffin: RPA70 Antibody [NBP2-20206]

Immunohistochemistry-Paraffin: RPA70 Antibody [NBP2-20206]

Immunohistochemistry-Paraffin: RPA70 Antibody [NBP2-20206] - Immunohistochemical analysis of paraffin-embedded SW480 Xenograft, using antibody at 1:100 dilution.

Applications for RPA70 Antibody

Application
Recommended Usage

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:1000-1:10000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: RPA70

Plays an essential role in several cellular processes in DNA metabolism including replication, recombination and DNA repair. Binds and subsequently stabilizes single-stranded DNA intermediates and thus prevents complementary DNA from reannealing. Functions as component of the alternative replication protein A complex (aRPA). aRPA binds single-stranded DNA and probably plays a role in DNA repair; it does not support chromosomal DNA replication and cell cycle progression through S-phase. In vitro, aRPA cannot promote efficient priming by DNA polymerase alpha but supports DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange.

Alternate Names

HSSB, MST075, MSTP075, REPA1RF-A protein 1, Replication factor A protein 1, replication protein A 70 kDa DNA-binding subunit, replication protein A1 (70kD), replication protein A1, 70kDa, RF-A, RP-A, RPA70RP-A p70, Single-stranded DNA-binding protein

Gene Symbol

RPA1

UniProt

P27694 (Human)

Additional RPA70 Products

Product Documents for RPA70 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for RPA70 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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