RPB8 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-80816

Novus Biologicals, part of Bio-Techne
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NBP1-80816
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Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all RPB8 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: AGILFEDIFDVKDIDPEGKKFDRVSRLHCESESFKMDLILDVNIQIYPVDLGDKFRLVIASTLYEDGTLDDGEY

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RPB8 Antibody

Western Blot: RPB8 Antibody [NBP1-80816]

Western Blot: RPB8 Antibody [NBP1-80816]

Western Blot: RPB8 Antibody [NBP1-80816] - Analysis using Anti-POLR2H antibody NBP1-80816 (A) shows similar pattern to independent antibody NBP2-76548 (B).
Immunohistochemistry-Paraffin: RPB8 Antibody [NBP1-80816]

Immunohistochemistry-Paraffin: RPB8 Antibody [NBP1-80816]

Immunohistochemistry-Paraffin: RPB8 Antibody [NBP1-80816] - Staining in human thyroid gland and pancreas tissues using anti-POLR2H antibody. Corresponding POLR2H RNA-seq data are presented for the same tissues.
Western Blot: RPB8 Antibody [NBP1-80816]

Western Blot: RPB8 Antibody [NBP1-80816]

Western Blot: RPB8 Antibody [NBP1-80816] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
View 2 more images

Applications for RPB8 Antibody

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:2500-1:5000

Western Blot

0.04 - 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RPB8

RPB8 encodes one of the essential subunits of RNA polymerase II that is shared by the other two eukaryotic DNA-directed RNA polymerases, I and III.

Alternate Names

DNA-directed RNA polymerases I, II, and III subunit RPABC3, hsRPB8, II, and III 17.1 kDa polypeptide, II, and III subunit ABC3, polymerase (RNA) II (DNA directed) polypeptide H, RPB8 homolog

Entrez Gene IDs

5437 (Human)

Gene Symbol

POLR2H

UniProt

P52434 (Human)

Additional RPB8 Products

Product Documents for RPB8 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for RPB8 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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