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RPL21 Antibody - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-93190

Novus Biologicals, part of Bio-Techne
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NBP2-93190-0.02ml
NBP2-93190-0.1ml

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Summary for RPL21 Antibody - Azide and BSA Free

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-160 of human RPL21 (NP_000973.2). MTNTKGKRRGTRYMFSRPFRKHGVVPLATYMRIYKKGDIVDIKGMGTVQKGMPHKCYHGKTGRVYNVTQHAVGIVVNKQVKGKILAKRINVRIEHIKHSKSRDSFLKRVKENDQKKKEAKEKGTWVQLKRQPAPPREAHFVRTNGKEPELLEPIPYEFMA

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

18 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for RPL21 Antibody - Azide and BSA Free

Western Blot: RPL21 AntibodyAzide and BSA Free [NBP2-93190]

Western Blot: RPL21 AntibodyAzide and BSA Free [NBP2-93190]

Western Blot: RPL21 Antibody [NBP2-93190] - Analysis of extracts of various cell lines, using RPL21 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 60s.
Immunocytochemistry/ Immunofluorescence: RPL21 Antibody - Azide and BSA Free [NBP2-93190]

Immunocytochemistry/ Immunofluorescence: RPL21 Antibody - Azide and BSA Free [NBP2-93190]

Immunocytochemistry/Immunofluorescence: RPL21 Antibody [NBP2-93190] - Analysis of HeLa cells using RPL21 at dilution of 1:200. Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: RPL21 Antibody - Azide and BSA Free [NBP2-93190]

Immunohistochemistry-Paraffin: RPL21 Antibody - Azide and BSA Free [NBP2-93190]

Immunohistochemistry-Paraffin: RPL21 Antibody [NBP2-93190] - Paraffin-embedded rat brain using RPL21 .

Applications for RPL21 Antibody - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:100

Immunohistochemistry

1:50-1:100

Western Blot

1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

Azide and BSA Free

Preservative

0.01% Thimerosal

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: RPL21

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L21E family of ribosomal proteins. It is located in the cytoplasm. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq]

Alternate Names

DKFZp686C06101, FLJ27458, L21,60S ribosomal protein L21, MGC104274, MGC104275, MGC71252, ribosomal protein L21

Gene Symbol

RPL21

Additional RPL21 Products

Product Documents for RPL21 Antibody - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for RPL21 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov