Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all RPL23A Antibodies »

Product Specifications

Immunogen

The immunogen this antibody was made to, maps to a region between residue 106 and 156 of human Ribosomal Protein L23a using the numbering given in entry NP_000975.2 (GeneID 6147).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RPL23A Antibody

Western Blot: RPL23A Antibody [NBP2-22284]

Western Blot: RPL23A Antibody [NBP2-22284]

Western Blot: RPL23A Antibody [NBP2-22284] - Whole cell lysate from HeLa (15 and 50 ug), 293T (50 ug), and Jurkat (50 ug) cells. NBP2-22284 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunoprecipitation: RPL23A Antibody [NBP2-22284]

Immunoprecipitation: RPL23A Antibody [NBP2-22284]

Immunoprecipitation: RPL23A Antibody [NBP2-22284] - Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: NBP2-22284 used for IP at 6 ug/mg lysate. RPL23A was also immunoprecipitated by rabbit anti-RPL23A antibody. For blotting immunoprecipitated RPL23A, NBP2-22284 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for RPL23A Antibody

Application
Recommended Usage

Immunoprecipitation

2 - 10 ug/mg

Western Blot

1:2000 - 1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: RPL23A

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L23P family of ribosomal proteins. It is located in the cytoplasm. The protein may be one of the target molecules involved in mediating growth inhibition by interferon. In yeast, the corresponding protein binds to a specific site on the 26S rRNA. This gene is co-transcribed with the U42A, U42B, U101A, and U101B small nucleolar RNA genes, which are located in its third, first, second, and fourth introns, respectively. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq]

Alternate Names

60S ribosomal protein L23a, FLJ27455, MDA20, melanoma differentiation-associated gene 20, ribosomal protein L23a

Gene Symbol

RPL23A

UniProt

NP_000975.2 (Human)

Additional RPL23A Products

Product Documents for RPL23A Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for RPL23A Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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