RPL29 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-12600
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Produced in rabbits immunized with E. coli-derived Human RPL29 fragment
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Protein A & Antigen Affinity purified
Protein A & Antigen Affinity purified
Scientific Data Images for RPL29 Antibody
Western Blot: RPL29 Antibody [NBP3-12600]
Western Blot: RPL29 Antibody [NBP3-12600] - Lane A: PC-3 Whole Cell LysateLane B: U87-MG Whole Cell LysateLane C: SW480 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:18 kDaObserved band size:22 kDaImmunocytochemistry/ Immunofluorescence: RPL29 Antibody [NBP3-12600]
Immunocytochemistry/Immunofluorescence: RPL29 Antibody [NBP3-12600] - Staining of RPL29 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human RPL29 polyclonal antibody (dilution ratio 1:200) at 4? overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.Immunohistochemistry-Paraffin: RPL29 Antibody [NBP3-12600]
Immunohistochemistry-Paraffin: RPL29 Antibody [NBP3-12600] - Staining of human RPL29 in human liver with rabbit polyclonal antibody at 1:10000 dilution.Applications for RPL29 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:500
Immunohistochemistry-Paraffin
1:2000-1:20000
Immunoprecipitation
1-5uL/mg of lysate
Western Blot
1:500-1:2000
Formulation, Preparation, and Storage
Purification
Antigen and protein A Affinity-purified
Formulation
PBS
Preservative
0.03% Proclin 300
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: RPL29
Alternate Names
60S ribosomal protein L29, Cell surface heparin-binding protein HIP, heparin/heparan sulfate-binding protein, heparin/heparan sulfate-interacting protein, HIPHUMRPL29, HP/HS-interacting protein, MGC88589, ribosomal protein L29
Gene Symbol
RPL29
Additional RPL29 Products
Product Specific Notices for RPL29 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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