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RPL35 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-81330

Novus Biologicals, part of Bio-Techne
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NBP1-81330
NBP1-81330-25ul

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (98%), Rat (98%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: AKIKARDLRGKKKEELLKQLDDLKVELSQLRVAKVTGGAASKLSKIRVVRKSIARVLTVINQTQKENLRKFYKGKKYKPLDLRPKKTRAMRRRLNKHEENLKTKKQQRKERLYPLRKYAVK

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RPL35 Antibody

Western Blot: RPL35 Antibody [NBP1-81330]

Western Blot: RPL35 Antibody [NBP1-81330]

Western Blot: RPL35 Antibody [NBP1-81330] - Analysis in human cell line MCF-7.
Immunocytochemistry/ Immunofluorescence: RPL35 Antibody [NBP1-81330]

Immunocytochemistry/ Immunofluorescence: RPL35 Antibody [NBP1-81330]

Immunocytochemistry/Immunofluorescence: RPL35 Antibody [NBP1-81330] - Staining of human cell line U-2 OS shows localization to cytosol & endoplasmic reticulum. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: RPL35 Antibody [NBP1-81330]

Immunohistochemistry-Paraffin: RPL35 Antibody [NBP1-81330]

Immunohistochemistry-Paraffin: RPL35 Antibody [NBP1-81330] - Staining of human skin shows weak cytoplasmic positivity in squamous epithelial cells.

Applications for RPL35 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04 - 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RPL35

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L29P family of ribosomal proteins. It is located in the cytoplasm. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. (provided by RefSeq)

Alternate Names

M, ribosomal protein L35,60S ribosomal protein L35

Gene Symbol

RPL35

Additional RPL35 Products

Product Documents for RPL35 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for RPL35 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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