RPL8 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-68143

Novus Biologicals, part of Bio-Techne
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NB100-68143
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Canine (100%), Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all RPL8 Antibodies »

Product Specifications

Immunogen

Peptide with sequence KAGRAYHKYKAKRNC corresponding to internal region according to NP_150644.1.

Epitope

KAGRAYHKYKAKRNC

Specificity

The reported variants represent identical protein (NP_000964.1 and NP_150644.1).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for RPL8 Antibody

Western Blot: RPL8 Antibody [NB100-68143]

Western Blot: RPL8 Antibody [NB100-68143]

Western Blot: RPL8 Antibody [NB100-68143] - Staining of HepG2 cell lysate (35 ug protein in RIPA buffer). Antibody at 0.01 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: RPL8 Antibody [NB100-68143]

Immunohistochemistry-Paraffin: RPL8 Antibody [NB100-68143]

Immunohistochemistry-Paraffin: RPL8 Antibody [NB100-68143] - Staining of paraffin embedded Human Spleen. Antibody at 2.5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunohistochemistry-Paraffin: RPL8 Antibody [NB100-68143]

Immunohistochemistry-Paraffin: RPL8 Antibody [NB100-68143]

Immunohistochemistry-Paraffin: RPL8 Antibody [NB100-68143] - Staining of paraffin embedded Human Cerebral Cortex. Antibody at 2.5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for RPL8 Antibody

Application
Recommended Usage

Immunohistochemistry

2 - 4 ug/mL

Immunohistochemistry-Paraffin

2 - 4 ug/mL

Peptide ELISA

Detection limit 1:128000

Western Blot

0.01 - 0.03 ug/mL
Application Notes
WB: Approx. 28-30 kDa band observed in HepG2 lysates (calculated MW of 28.0 kDa band according to NP_000964.1 and NP_150644.1).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: RPL8

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L2P family of ribosomal proteins. It is located in the cytoplasm. In rat, the protein associates with the 5.8S rRNA, very likely participates in the binding of aminoacyl-tRNA, and is a constituent of the elongation factor 2-binding site at the ribosomal subunit interface. Alternatively spliced transcript variants encoding the same protein exist. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.

Alternate Names

ribosomal protein L8,60S ribosomal protein L8

Entrez Gene IDs

6132 (Human); 26961 (Mouse); 26962 (Rat)

Gene Symbol

RPL8

UniProt

NP_000964.1 (Human)

Additional RPL8 Products

Product Documents for RPL8 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for RPL8 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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