RPP38 Antibody - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-94050
Key Product Details
Species Reactivity
Validated:
Human
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
Azide and BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for RPP38 Antibody - Azide and BSA Free
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 20-110 of human RPP38 (NP_892117.1). VVKTSLNNPYIIRWSALESEDMHFILQTLEDRLKAIGLQKIEDKKKKNKTPFLKKESREKCSIAVDISENLKEKKTDAKQQVSGWTPAHVR
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
31 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for RPP38 Antibody - Azide and BSA Free
Western Blot: RPP38 AntibodyAzide and BSA Free [NBP2-94050]
Western Blot: RPP38 Antibody [NBP2-94050] - Analysis of extracts of various cell lines, using RPP38 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 30s.Immunocytochemistry/ Immunofluorescence: RPP38 Antibody - Azide and BSA Free [NBP2-94050]
Immunocytochemistry/Immunofluorescence: RPP38 Antibody [NBP2-94050] - Analysis of U-2 OS cells using RPP38 at dilution of 1:100. Blue: DAPI for nuclear staining.Immunohistochemistry-Paraffin: RPP38 Antibody - Azide and BSA Free [NBP2-94050]
Immunohistochemistry-Paraffin: RPP38 Antibody [NBP2-94050] - Immunohistochemistry of paraffin-embedded Human lung cancer using RPP38 Rabbit pAb (NBP2-94050) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.Applications for RPP38 Antibody - Azide and BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50-1:200
Immunohistochemistry
1:50-1:200
Western Blot
1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol
Format
Azide and BSA Free
Preservative
0.01% Thimerosal
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: RPP38
Alternate Names
EC 3.1.26.5, ribonuclease P (38kD) (RPP38), ribonuclease P protein subunit p38, ribonuclease P/MRP 38kDa subunit, RNaseP protein p38
Gene Symbol
RPP38
Additional RPP38 Products
Product Documents for RPP38 Antibody - Azide and BSA Free
Product Specific Notices for RPP38 Antibody - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov