RPP40 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-88765

Novus Biologicals, part of Bio-Techne
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NBP1-88765
NBP1-88765-25ul
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all RPP40 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: RALELFDWLGAVFSNVDLNNEPNNFISTYCCPEPSTVVAKAYLCTITGFILPEKICLLLEHLCHYFDEPKLAPWVTLSVQ

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (83%), Rat (81%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RPP40 Antibody

Western Blot: RPP40 Antibody [NBP1-88765]

Western Blot: RPP40 Antibody [NBP1-88765]

Western Blot: RPP40 Antibody [NBP1-88765] - Analysis in control (vector only transfected HEK293T lysate) and RPP40 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: RPP40 Antibody [NBP1-88765]

Immunocytochemistry/ Immunofluorescence: RPP40 Antibody [NBP1-88765]

Immunocytochemistry/Immunofluorescence: RPP40 Antibody [NBP1-88765] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
Immunohistochemistry-Paraffin: RPP40 Antibody [NBP1-88765]

Immunohistochemistry-Paraffin: RPP40 Antibody [NBP1-88765]

Immunohistochemistry-Paraffin: RPP40 Antibody [NBP1-88765] - Staining of human cerebral cortex shows cytoplasmic positivity in neuronal cells.

Applications for RPP40 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RPP40

RPP40 is a component of ribonuclease P, a protein complex that generates mature tRNA molecules by cleaving their 5'-ends

Alternate Names

bA428J1.3,40kD subunit, EC 3.1.26.5, ribonuclease P/MRP 40kDa subunit, RNaseP protein p40

Gene Symbol

RPP40

Additional RPP40 Products

Product Documents for RPP40 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for RPP40 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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