RPS20 Antibody (9H1D4)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16824

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16824-100ul
NBP3-16824-20ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 9H1D4

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all RPS20 Antibodies »

Product Summary for RPS20 Antibody (9H1D4)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human RPS20 (P60866). MAFKDTGKTPVEPEVAIHRIRITLTSRNVKSLEKVCADLIRGAKEKNLKVKGPVRMPTKTLRITTRKTPCGEGSKTWDRFQMRIHKRLIDLHSPSEIVKQ

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RPS20 Antibody (9H1D4)

Western Blot: RPS20 Antibody (9H1D4) [NBP3-16824]

Western Blot: RPS20 Antibody (9H1D4) [NBP3-16824]

Western Blot: RPS20 Antibody (9H1D4) [NBP3-16824] - Western blot analysis of extracts of various cell lines, using RPS20 Rabbit mAb (NBP3-16824) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.
Immunocytochemistry/ Immunofluorescence: RPS20 Antibody (9H1D4) [NBP3-16824]

Immunocytochemistry/ Immunofluorescence: RPS20 Antibody (9H1D4) [NBP3-16824]

Immunocytochemistry/Immunofluorescence: RPS20 Antibody (9H1D4) [NBP3-16824] - Immunofluorescence analysis of C6 cells using RPS20 Rabbit mAb (NBP3-16824) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: RPS20 Antibody (9H1D4) [NBP3-16824]

Immunohistochemistry-Paraffin: RPS20 Antibody (9H1D4) [NBP3-16824]

Immunohistochemistry-Paraffin: RPS20 Antibody (9H1D4) [NBP3-16824] - Immunohistochemistry of paraffin-embedded mouse brain using RPS20 Rabbit mAb (NBP3-16824) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Applications for RPS20 Antibody (9H1D4)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: RPS20

Mammalian ribosomal proteins are encoded by multigene families that consist of processed pseudogenes and one functional intron-containing gene within their coding regions. Ribosomal Protein S20, also known as RPS20, is a 119 amino acid cytoplasmic protein that is a component of the 40S ribosomal subunit. Co-transcribed with the small nucleolar RNA gene U54, Ribosomal Protein S20 is a primary binding protein (it binds independently to its target protein) that interacts with both the 5' and 3' minor domains of 16S ribosomal RNA (rRNA). Through its interactions with 16S rRNA, Ribosomal Protein S20 is thought to play a key role in nucleating the assembly of the 30S ribosomal subunit. Like most ribosomal protein-coding genes, the gene encoding Ribosomal Protein S20 is dispersed throughout the genome and exists as multiple processed pseudogenes.

Alternate Names

40S ribosomal protein S20, FLJ27451, MGC102930, ribosomal protein S20

Gene Symbol

RPS20

Additional RPS20 Products

Product Documents for RPS20 Antibody (9H1D4)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for RPS20 Antibody (9H1D4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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