RPTOR Antibody (JE59-39)

Western Blot: RPTOR Antibody (JE59-39) [NBP3-32894]

Western Blot: RPTOR Antibody (JE59-39) [NBP3-32894] - Western blot analysis of RPTOR on different lysates with Rabbit anti-RPTOR antibody (NBP3-32894) at 1/2,000 dilution.

Lane 1: 293T cell lysate (30 ug/Lane)
Lane 2: K-562 cell lysate (30 ug/Lane)
Lane 3: Neuro-2a cell lysate (30 ug/Lane)
Lane 4: Mouse brain tissue lysate (40 ug/Lane)
Lane 5: Rat brain tissue lysate (40 ug/Lane)

Predicted band size: 149 kDa
Observed band size: 149 kDa

Exposure time: 3 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32894) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: RPTOR Antibody (JE59-39) [NBP3-32894]

Immunohistochemistry: RPTOR Antibody (JE59-39) [NBP3-32894] - Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-RPTOR antibody (NBP3-32894) at 1/500 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32894) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.