RPTOR Antibody (SR1170)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-21541

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-21541-100ul
NBP3-21541-50ul
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # SR1170

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all RPTOR Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human RPTOR (Uniprot #: Q8N122)

Specificity

Detects endogenous levels of RPTOR

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

149 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for RPTOR Antibody (SR1170)

Western Blot: RPTOR Antibody (SR1170) [NBP3-21541] -

Western Blot: RPTOR Antibody (SR1170) [NBP3-21541] - Western blot analysis using NBP3-21541 in MCF-7 cell lysate.

Applications for RPTOR Antibody (SR1170)

Application
Recommended Usage

Flow Cytometry

1:30

Immunocytochemistry/ Immunofluorescence

1:50-1:100

Immunohistochemistry

1:50-1:100

Immunoprecipitation

1:30

Western Blot

1:500-1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, 50% glycerol.

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RPTOR

Raptor (regulatory associated protein of TOR) is a conserved 150 kDa adaptor protein composed of a unique conserved region, three HEAT repeats and seven WD-40 repeats (1). Raptor recruits mTOR substrates S6K1 (p70 S6 kinase) and 4E-BP1, and regulates mTOR kinase activity (2-3). Even though Raptor is a positive regulator of mTOR, it has been shown that under nutrient deprivation, the Raptor-mTOR association is stabilized in a manner that inhibits mTOR kinase activity (1). Raptor association with mTOR is required for efficient S6K1 and 4E0BP1 phosphorylation (4).

Long Name

Regulatory Associated Protein of mTOR

Alternate Names

KIAA1303, Raptor

Gene Symbol

RPTOR

Additional RPTOR Products

Product Documents for RPTOR Antibody (SR1170)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for RPTOR Antibody (SR1170)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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