RPTOR Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-77211

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Mouse

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all RPTOR Antibodies »

Product Specifications

Immunogen

Antibody was raised against a 13 amino acid synthetic peptide from near the amino-terminus of human Raptor. The immunogen is located within amino acids 90 - 140 of Raptor. Amino Acid Squence: KQYENWQPRARYK

Specificity

Raptor has multiple isoforms that may also be recognized by antibody.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RPTOR Antibody - BSA Free

Western Blot: RPTOR AntibodyBSA Free [NBP1-77211]

Western Blot: RPTOR AntibodyBSA Free [NBP1-77211]

Western Blot: RPTOR Antibody [NBP1-77211] - Analysis of RAPTOR expression in Jurkat cell lysate with RAPTOR antibody at (A) 1 and (B) 2 ug/ml.
Immunocytochemistry/ Immunofluorescence: RPTOR Antibody - BSA Free [NBP1-77211]

Immunocytochemistry/ Immunofluorescence: RPTOR Antibody - BSA Free [NBP1-77211]

Immunocytochemistry/Immunofluorescence: RPTOR Antibody [NBP1-77211] - Immunocytochemistry of RAPTOR in L1210 cells with RAPTOR antibody at 10 ug/mL.
Immunocytochemistry/ Immunofluorescence: RPTOR Antibody - BSA Free [NBP1-77211]

Immunocytochemistry/ Immunofluorescence: RPTOR Antibody - BSA Free [NBP1-77211]

Immunocytochemistry/Immunofluorescence: RPTOR Antibody [NBP1-77211] - L1210 cells at 10 ug/mL.

Applications for RPTOR Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

10 ug/ml

Western Blot

2-4 ug/ml

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RPTOR

The mammalian Target of Rapamycin (TOR, also known as mTOR) is an evolutionarily conserved serine/threonine kinase that regulates cell growth and cell cycle through its ability to integrate signals from nutrient levels and growth factors. Rapamycin inhibits TOR activity resulting in reduced cell growth and reduced rates of cell cycle and cell proliferation. Raptor (regulatory associated protein of TOR) is a TOR-binding protein essential for TOR signaling in vivo. It acts as a TOR scaffold protein whose binding by TOR substrates is necessary for effective TOR-catalyzed phosphorylation. These substrates include the ribosomal protein S6 kinase (RP S6K) and the eukaryotic initiation factor 4E binding protein 4EBP1, proteins necessary for cell growth and proliferation and responsive to nutrient and mitogen levels. Raptor binds these proteins through a common 5 amino acid TOR-signaling (TOS) motif; mutation of this motif prevents the TOR-dependent phosphorylation of these proteins.

Long Name

Regulatory Associated Protein of mTOR

Alternate Names

KIAA1303, Raptor

Gene Symbol

RPTOR

UniProt

Q8N122 (Human)

Additional RPTOR Products

Product Documents for RPTOR Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for RPTOR Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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