RRAD Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-27500
Key Product Details
Species Reactivity
Validated:
Mouse
Predicted:
Bovine (100%), Canine (100%), Human (100%), Porcine (100%), Rat (100%). Backed by our 100% Guarantee.
Applications
Knockout Validated, Peptide ELISA, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Peptide with sequence C-HRRSMPVDERDLQ corresponding to internal region according to NP_004156.1.
Specificity
Reported variants represent identical protein: NP_004156.1, NP_001122322.1
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for RRAD Antibody
![Western Blot: RRAD Antibody [NBP2-27500]](https://resources.bio-techne.com/images/products/RRAD-Antibody-Western-Blot-NBP2-27500-img0003.jpg "Western Blot: RRAD Antibody [NBP2-27500]")
Western Blot: RRAD Antibody [NBP2-27500]
Western Blot: RRAD Antibody [NBP2-27500] - Panel A: Staining of HEK293 lysates overexpressing several HA-tagged Mouse GTPases, including Rrad (10 ug protein in RIPA buffer) with antibody at 0.5 ug/mL, and compared with an HA-specific antibody. Panel B: Staining of WT and KO lysates of Mouse Heart (100 ug protein in RIPA buffer) with antibody at 0.5 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence. Data provided by Prof. D Andres, University of Kentucky College of Medicine, USA.Applications for RRAD Antibody
Application
Recommended Usage
Peptide ELISA
Detection limit 1:64000
Western Blot
0.5 - 2 ug/mL
Application Notes
WB: Approx. 37 kDa band observed in mouse heart lysates and no 37 kDa band in the KO lysates (calculated MW of 33.2 kDa band according to mouse NP_062636.2 and human NP_004156.1). There is some non-specific background, we call for caution when used for other assays than WB. In transfected HEK293 transiently expressing mouse Rrad (HA tagged), a band of approx. 45 kDa is observed. No bands are observed in transfected HEK293 transiently expressing related mouse GTPases.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: RRAD
Alternate Names
RAD1, RADGTP-binding protein RAD, RAS (RAD and GEM) like GTP binding 3, Ras associated with diabetes, Ras-related associated with diabetes, REM3
Gene Symbol
RRAD
UniProt
Additional RRAD Products
Product Documents for RRAD Antibody
Product Specific Notices for RRAD Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...