RRAS Antibody (2E12)

Novus Biologicals, part of Bio-Techne | Catalog # H00006237-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 2E12

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all RRAS Antibodies »

Product Specifications

Immunogen

RRAS (AAH16286, 109 a.a. ~ 218 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. AINDRQSFNEVGKLFTQILRVKDRDDFPVVLVGNKADLESQRQVPRSEASAFGASHHVAYFEASAKLRLNVDEAFEQLVRAVRKYQEQELPPSPPSAPRKKGGGCPCVLL

Specificity

RRAS - related RAS viral (r-ras) oncogene homolog

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for RRAS Antibody (2E12)

Western Blot: RRAS Antibody (2E12) [H00006237-M01]

Western Blot: RRAS Antibody (2E12) [H00006237-M01]

Western Blot: RRAS Antibody (2E12) [H00006237-M01] - RRAS monoclonal antibody (M01), clone 2E12 Analysis of RRAS expression in HeLa.
Immunoprecipitation: RRAS Antibody (2E12) [H00006237-M01]

Immunoprecipitation: RRAS Antibody (2E12) [H00006237-M01]

Immunoprecipitation: RRAS Antibody (2E12) [H00006237-M01] - Analysis of RRAS transfected lysate using anti-RRAS monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with RRAS MaxPab rabbit polyclonal antibody.
ELISA: RRAS Antibody (2E12) [H00006237-M01]

ELISA: RRAS Antibody (2E12) [H00006237-M01]

ELISA: RRAS Antibody (2E12) [H00006237-M01] - RRAS Antibody (2E12) [H00006237-M01] - Detection limit for recombinant GST tagged RRAS is approximately 0.3ng/ml as a capture antibody.

Applications for RRAS Antibody (2E12)

Application
Recommended Usage

Immunoprecipitation

1:10-1:500

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: RRAS

R-Ras is a member of the Ras superfamily of GTPases. Ras proteins function as signaling focal points that are activated by convergent signaling pathways intiated by extracellular stimuli (reviewed in Mitin et al, 2005). Activated Ras proteins in turn regulate a diversity of downstream cytoplasmic signaling cascades. Many members of the Ras superfamily are implicated in the regulation of hematopoietic cells, with roles in growth, survival, differentiation, cytokine production, chemotaxis, vesicle-trafficking, and phagocytosis. The Ras superfamily of proteins includes over 150 small GTPases (distinguished from the large, heterotrimeric GTPases, the G-proteins). It comprises six subfamilies, the Ras, Rho, Ran, Rab, Arf, and Kir/Rem/Rad subfamilies. They exhibit considerable overall amino acid identities, especially in the regions interacting with the guanine nucleotide exchange factors that catalyze their activation. Recognizes R-Ras; human R-Ras is a 218 amino acid protein.

Alternate Names

Oncogene RRAS, p23, ras-related protein R-Ras, related RAS viral (r-ras) oncogene homolog

Entrez Gene IDs

6237 (Human)

Gene Symbol

RRAS

OMIM

165090 (Human)

UniProt

AAH16286 (Human)

Additional RRAS Products

Product Documents for RRAS Antibody (2E12)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for RRAS Antibody (2E12)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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