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RSF1 Antibody (C.15200041)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-59285

Novus Biologicals, part of Bio-Techne
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NBP2-59285

Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # C.15200041

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Summary for RSF1 Antibody (C.15200041)

Immunogen

The exact immunogen is propietary information.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for RSF1 Antibody (C.15200041)

Western Blot: RSF1 Antibody (C.15200041) [NBP2-59285]

Western Blot: RSF1 Antibody (C.15200041) [NBP2-59285]

Western Blot: RSF1 Antibody (C.15200041) [NBP2-59285] - Analysis of nuclear extracts from HeLa cells (HeLa NE, 20 ug) using the monoclonal antibody directed against hRSF1, diluted 1:500 in TBS-Tween containing 5% skimmed milk. The molecular weight (in kDa) is shown on the left, the position of the protein of interest is shown on the right.

Applications for RSF1 Antibody (C.15200041)

Application
Recommended Usage

Immunoprecipitation

0.3 ug

Western Blot

1:500
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: RSF1

RSF1 (UniProtKB/Swiss-Prot entry Q96T23) is required for assembly of regular nucleosome arrays by the RSF chromatin remodelling complex. RSF1 facilitates transcription of hepatitis B virus (HBV) genes by the pX transcription activator. In case of infection by HBV, together with pX, it represses TNF-alpha induced NF-kappaB transcription activation. RSF1 represses transcription when artificially recruited to chromatin by fusion to a heterogeneous DNA binding domain. RSF1 interacts with SMARCA5/SNF2H to form the RSF complex and also binds the HBV pX/HBx protein, which is required to activate transcription of the viral genome.

Alternate Names

HBV pX associated protein-8, HBXAPp325 subunit of RSF chromatin-remodeling complex, hepatitis B virus x associated protein, Hepatitis B virus X-associated protein, p325, remodeling and spacing factor 1, Rsf-1, XAP8HBV pX-associated protein 8

Gene Symbol

RSF1

Additional RSF1 Products

Product Documents for RSF1 Antibody (C.15200041)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for RSF1 Antibody (C.15200041)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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