RSK1 Antibody (S02-2D2)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-20053

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-20053-100ul
NBP3-20053-50ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # S02-2D2

Concentration

0.3 mg/ml

View all RSK1 Antibodies »

Product Summary for RSK1 Antibody (S02-2D2)

Immunogen

A synthetic peptide of human RSK1 (Uniprot # Q15418)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

90 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for RSK1 Antibody (S02-2D2)

Western Blot: RSK1 Antibody (S02-2D2) [NBP3-20053]

Western Blot: RSK1 Antibody (S02-2D2) [NBP3-20053]

Western Blot: RSK1 Antibody (S02-2D2) [NBP3-20053] - Western blot detection of RSK1 in Hela cell lysates using NBP3-20053 (1:500 diluted). Predicted band size: 83kDa. Observed band size: 90kDa.
Immunohistochemistry-Paraffin: RSK1 Antibody (S02-2D2) [NBP3-20053]

Immunohistochemistry-Paraffin: RSK1 Antibody (S02-2D2) [NBP3-20053]

Immunohistochemistry-Paraffin: RSK1 Antibody (S02-2D2) [NBP3-20053] - Immunohistochemistry of RSK1 in paraffin-embedded Human Cholangiocarcinoma using NBP3-20053 at dilution 1/50

Applications for RSK1 Antibody (S02-2D2)

Application
Recommended Usage

Immunohistochemistry

1/20

Western Blot

1/1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA

Preservative

0.01% Sodium Azide

Concentration

0.3 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RSK1

Rsk-1 (p90-Rsk-1) is a member of a family of 90 kDa ribosomal protein S6 kinases, which includes Rsk-1, Rsk-2 and Rsk-3. These are broadly expressed serine/threonine protein kinases (1) activated in response to mitogenic stimuli, including extracellular signal-regulated protein kinases Erk1 and Erk2 (2). p90-Rsk-1 is activated by MAPK in vitro and in vivo via phosphorylation (3). Active Rsks appear to play a major role in transcriptional regulation by translocating to the nucleus and phosphorylating c-Fos and CREB (2,4).

Long Name

Ribosomal Protein S6 Kinase I

Alternate Names

HU-1, MAPKAPL1A, RPS6KA1

Gene Symbol

RPS6KA1

Additional RSK1 Products

Product Documents for RSK1 Antibody (S02-2D2)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for RSK1 Antibody (S02-2D2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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