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RTF1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-61052

Novus Biologicals, part of Bio-Techne
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NB100-61052

Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.2 mg/ml

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to the C-terminus of human Restores TBP (TATA box-binding protein) Function-1 using the numbering given in entry EAW92492.1 (GeneID 23168).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RTF1 Antibody

Western Blot: RTF1 Antibody [NB100-61052]

Western Blot: RTF1 Antibody [NB100-61052]

Western Blot: RTF1 Antibody [NB100-61052] - Detection of Human RTF1 on HeLa whole cell lysate using NB100-61052. RTF1 was also immunoprecipitated by rabbit anti-RTF1 antibody NB600-178.
Immunohistochemistry-Paraffin: RTF1 Antibody [NB100-61052]

Immunohistochemistry-Paraffin: RTF1 Antibody [NB100-61052]

Immunohistochemistry-Paraffin: RTF1 Antibody [NB100-61052] - Sample: FFPE section of human skin carcinoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti- RTF1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB

Applications for RTF1 Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:100-1:500

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: RTF1

In yeast, Rtf1 is a component of the Paf1/RNA polymerase II complex. The Paf1 complex interacts with RNA polymerase II and functions to modify chromatin during transcription. Rtf1 appears to be important for histone H2B ubiquitination by Rad6 and for methylation of histone H3 catalyzed by the Set1/COMPASS complex that functions during transcription elongation.

Alternate Names

KIAA0252GTL7, ortholog of mouse gene trap locus 7, RNA polymerase-associated protein RTF1 homolog, Rtf1, Paf1/RNA polymerase II complex component, homolog (S. cerevisiae)

Entrez Gene IDs

23168 (Human)

Gene Symbol

RTF1

Additional RTF1 Products

Product Documents for RTF1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for RTF1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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