Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all RTF1 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 620 and 670 of human Restores TBP (TATA box-binding protein) function-1 using the numbering given in entry EAW92492.1 (GeneID 23168).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RTF1 Antibody

Western Blot: RTF1 Antibody [NB600-278]

Western Blot: RTF1 Antibody [NB600-278]

Western Blot: RTF1 Antibody [NB600-278] - Detection of Human Rtf1 by Western Blot. Samples: Whole cell lysate (5, 15 and 50 ug) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Rtf1 antibody NB600-278 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunoprecipitation: RTF1 Antibody [NB600-278]

Immunoprecipitation: RTF1 Antibody [NB600-278]

Immunoprecipitation: RTF1 Antibody [NB600-278] - Detection of human Rtf1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Rtf1 antibody NB600-278 used for IP at 3 ug per reaction. Rtf1 was also immunoprecipitated by rabbit anti-RTF1 antibodies NB600-277 and NB100-61052. For blotting immunoprecipitated Rtf1, NB600-278 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Applications for RTF1 Antibody

Application
Recommended Usage

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:10000-1:30000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: RTF1

RTF1 is important for TATA site selection by TBP. It directly or indirectly regulates the DNA-binding properties of SPT15, the TATA box-binding protein, and the relative activities of different TATA elements.

Alternate Names

KIAA0252GTL7, ortholog of mouse gene trap locus 7, RNA polymerase-associated protein RTF1 homolog, Rtf1, Paf1/RNA polymerase II complex component, homolog (S. cerevisiae)

Entrez Gene IDs

23168 (Human)

Gene Symbol

RTF1

UniProt

EAW92492.1 (Human)

Additional RTF1 Products

Product Documents for RTF1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for RTF1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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