Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Hamster

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Cited:

Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # MON162

Format

BSA Free

Concentration

1 mg/ml

View all RTN1-A/NSP Antibodies »

Product Specifications

Immunogen

Derived by fusion of Mouse myeloma cells with spleen cells from a Mouse immunized with a partially purified bacterially expressed Reticulon-1A (NSP-A) hybrid protein.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Specificity

This antibody exclusively recognizes the 135 kD RTN1-A/NSP (RTN1-A/NSP) protein in immunoblots of NCI-H82 and other SCLC cell lines, and stains normal and pathological neural and neuroendocrine tissues. The epitope of MON-162 is located between amino acid residues 338-422 of RTN1-A/NSP.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Description

The antibody is shipped at ambient temperature and may be stored at 4C. For prolonged storage prepare appropriate aliquots and store at or below -20C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at 4C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product.

Scientific Data Images for RTN1-A/NSP Antibody (MON162) - BSA Free

Immunocytochemistry/ Immunofluorescence: RTN1-A/NSP Antibody (MON162) - BSA Free [NBP1-97677]

Immunocytochemistry/ Immunofluorescence: RTN1-A/NSP Antibody (MON162) - BSA Free [NBP1-97677]

Immunocytochemistry/Immunofluorescence: RTN1 Antibody (MON162) [NBP1-97677] - Immunostaining of NCI-H82 variant small cell lung cancer
Immunohistochemistry-Frozen: RTN1-A/NSP Antibody (MON162) - BSA Free [NBP1-97677]

Immunohistochemistry-Frozen: RTN1-A/NSP Antibody (MON162) - BSA Free [NBP1-97677]

Immunohistochemistry-Frozen: RTN1 Antibody (MON162) [NBP1-97677] - Immunohistochemistry on frozen section of swine brain

Applications for RTN1-A/NSP Antibody (MON162) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:100-1:200

Immunohistochemistry-Paraffin

1:100-1:200

Western Blot

1:100-1:1000
Application Notes
This antibody is suitable for immunocytochemistry, immunohistochemistry on frozen and paraffin-embedded tissue, and immunoblotting. Optimal antibody dilution should be determined by titration; recommended range is 1:100 - 1:200 for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting applications..

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS, pH 7.2

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RTN1-A/NSP

Long Name

Reticulon 1A/Neuroendocrine-specific Protein

Alternate Names

NSP-A, RTN1, RTN1A

Gene Symbol

RTN1

Additional RTN1-A/NSP Products

Product Documents for RTN1-A/NSP Antibody (MON162) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for RTN1-A/NSP Antibody (MON162) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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