RTN1-A/NSP Antibody (RNL-4) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-97673
![Western Blot: RTN1-A/NSP Antibody (RNL-4)BSA Free [NBP1-97673]](https://resources.bio-techne.com/images/products/RTN1-Antibody-1988-Western-Blot-NBP1-97673-img0001.jpg "Western Blot: RTN1-A/NSP Antibody (RNL-4)BSA Free [NBP1-97673]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Rat, Porcine
Applications
CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # RNL-4
Format
BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Derived by fusion of SP2/0-Ag14 Mouse myeloma cells, with spleen cells from a BALB/c Mouse immunized with a synthetic peptide encompassing the unique 20 N-terminal amino acid sequence of Reticulon-1C.
Specificity
This antibody recognizes an epitope located within the first 20 amino acids of Reticulon-1C (NSP-C). It reacts with peripheral nerves and ganglia of various tissues and cross-reacts with smooth muscle cells and myoepithelium. In the central nervous system it reacts with the neurohypophysis and pars intermedia of the pituitary gland, and a weak diffuse staining was observed in neurons of the granular and molecular layer of the cerebellar cortex, while glial cells, cerebellar medulla and Purkinje cells are negative. Reticulon-1 has been found to indicate neuronal differentiation and to be downregulated in neurological pathologies.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Description
The antibody is shipped at ambient temperature and may be stored at 4C. For prolonged storage prepare appropriate aliquots and store at or below -20C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at 4C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product.
Scientific Data Images for RTN1-A/NSP Antibody (RNL-4) - BSA Free
Western Blot: RTN1-A/NSP Antibody (RNL-4)BSA Free [NBP1-97673]
Western Blot: RTN1 Antibody (1988) [NBP1-97673] - Immunoblotting of RNL-4 recognizing NSP-C Reticulon 1-C (23 kDa) in extract from neuroblastoma cell line LA-N-5Applications for RTN1-A/NSP Antibody (RNL-4) - BSA Free
Application
Recommended Usage
Flow Cytometry
1:50-1:100
Immunocytochemistry/ Immunofluorescence
1:10-1:500
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Frozen
1:10-1:500
Western Blot
1:100-1:2000
Application Notes
Optimal antibody dilution should be determined by titration; recommended range is 1:50 - 1:100 for flow cytometry, and for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:500 for immunoblotting applications.
This antibody is CyTOF ready..
This antibody is CyTOF ready..
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
PBS, pH 7.2
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: RTN1-A/NSP
Long Name
Reticulon 1A/Neuroendocrine-specific Protein
Alternate Names
NSP-A, RTN1, RTN1A
Gene Symbol
RTN1
Additional RTN1-A/NSP Products
Product Documents for RTN1-A/NSP Antibody (RNL-4) - BSA Free
Product Specific Notices for RTN1-A/NSP Antibody (RNL-4) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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