RTN3 Antibody (PSH0-40)

Western Blot: RTN3 Antibody (PSH0-40) [NBP3-32921]

Western Blot: RTN3 Antibody (PSH0-40) [NBP3-32921] - Western blot analysis of RTN3 on different lysates with Rabbit anti-RTN3 antibody (NBP3-32921) at 1/1,000 dilution.

Lane 1: HeLa cell lysate (32 ug/Lane)
Lane 2: HEK-293 cell lysate (33 ug/Lane)
Lane 3: U87-MG cell lysate (30 ug/Lane)
Lane 4: A549 cell lysate (26 ug/Lane)
Lane 5: SH-SY5Y cell lysate (18 ug/Lane)
Lane 6: PC-12 cell lysate (23 ug/Lane)
Lane 7: L6 cell lysate (14 ug/Lane)
Lane 8: Human cerebellum tissue lysate (40 ug/Lane)

Lysates/proteins at 10 ug/Lane.

Predicted band size: 112.6 kDa
Observed band size: 100 kDa

Exposure time: 3 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32921) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry/ Immunofluorescence: RTN3 Antibody (PSH0-40) [NBP3-32921]

Immunocytochemistry/ Immunofluorescence: RTN3 Antibody (PSH0-40) [NBP3-32921] - Immunocytochemistry analysis of PC-12 cells labeling RTN3 with Rabbit anti-RTN3 antibody (NBP3-32921) at 1/50 dilution.

Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-RTN3 antibody (NBP3-32921) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.

Flow Cytometry: RTN3 Antibody (PSH0-40) [NBP3-32921]

Flow Cytometry: RTN3 Antibody (PSH0-40) [NBP3-32921] - Flow cytometric analysis of HeLa cells labeling RTN3.

Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32921, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).