RUFY1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-68865
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Peptide with sequence C-QKNEAITSFEGKT corresponding to internal region according to NP_001035541.1.
Epitope
C-QKNEAITSFEGKT
Reactivity Notes
Expected from sequence similarity: Mouse, Rat, Canine, Bovine
Specificity
This antibody is expected to recognize both reported isoforms (NP_079434.3; NP_001035541.1). Reported variants represent identical protein: NP_001035542.1, NP_001035541.1.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for RUFY1 Antibody
![Western Blot: RUFY1 Antibody [NBP1-68865]](https://resources.bio-techne.com/images/products/RUFY1-Antibody-Western-Blot-NBP1-68865-img0002.jpg "Western Blot: RUFY1 Antibody [NBP1-68865]")
Western Blot: RUFY1 Antibody [NBP1-68865]
Western Blot: RUFY1 Antibody [NBP1-68865] - NBP1-68865 (0.05ug/ml) staining of HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.![Immunohistochemistry-Paraffin: RUFY1 Antibody [NBP1-68865]](https://resources.bio-techne.com/images/products/RUFY1-Antibody-Immunohistochemistry-Paraffin-NBP1-68865-img0003.jpg "Immunohistochemistry-Paraffin: RUFY1 Antibody [NBP1-68865]")
Immunohistochemistry-Paraffin: RUFY1 Antibody [NBP1-68865]
Immunohistochemistry-Paraffin: RUFY1 Antibody [NBP1-68865] - 5ug/ml staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer Ph 6, AP-staining.Applications for RUFY1 Antibody
Application
Recommended Usage
Immunohistochemistry
0.05-0.2ug/ml
Peptide ELISA
Detection limit 1:32000
Western Blot
0.05-0.2 ug/ml
Application Notes
Western blot: Approx 85kDa band observed in lysates of cell line HeLa (calculated MW of 79.8kDa according to NP_079434.3). Primary incubation was 1 hour
IHC: Paraffin embedded Human Small Intestine.
IHC: Paraffin embedded Human Small Intestine.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: RUFY1
Alternate Names
La-binding protein 1, Rab4-interacting protein, RABIP4FYVE-finger protein EIP1, RUN and FYVE domain containing 1, RUN and FYVE domain-containing 1, RUN and FYVE domain-containing protein 1, ZFYVE12FLJ22251, Zinc finger FYVE domain-containing protein 12
Gene Symbol
RUFY1
UniProt
Additional RUFY1 Products
Product Documents for RUFY1 Antibody
Product Specific Notices for RUFY1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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