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RUFY1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-20241

Novus Biologicals, part of Bio-Techne
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NBP2-20241

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human RUFY1. The exact sequence is proprietary.

Localization

Cytoplasm; Early endosome membrane; Peripheral membrane protein

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

69 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for RUFY1 Antibody

Western Blot: RUFY1 Antibody [NBP2-20241]

Western Blot: RUFY1 Antibody [NBP2-20241]

Western Blot: RUFY1 Antibody [NBP2-20241] - Sample (30 ug of whole cell lysate) A: NT2D1 7. 5% SDS PAGE gel, diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: RUFY1 Antibody [NBP2-20241]

Immunocytochemistry/ Immunofluorescence: RUFY1 Antibody [NBP2-20241]

Immunocytochemistry/Immunofluorescence: RUFY1 Antibody [NBP2-20241] - Analysis of methanol-fixed MCF-7, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: RUFY1 Antibody [NBP2-20241]

Immunohistochemistry-Paraffin: RUFY1 Antibody [NBP2-20241]

Immunohistochemistry-Paraffin: RUFY1 Antibody [NBP2-20241] - RUFY1 antibody detects RUFY1 protein at cytoplasm on human breast cancer by immunohistochemical analysis. Sample: Paraffin-embedded breast cancer. RUFY1 antibody dilution: 1:250.

Applications for RUFY1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

10 - 1:500

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol, 1% BSA

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: RUFY1

Binds phospholipid vesicles containing phosphatidylinositol 3-phosphate and participates in early endosomal trafficking.

Alternate Names

La-binding protein 1, Rab4-interacting protein, RABIP4FYVE-finger protein EIP1, RUN and FYVE domain containing 1, RUN and FYVE domain-containing 1, RUN and FYVE domain-containing protein 1, ZFYVE12FLJ22251, Zinc finger FYVE domain-containing protein 12

Gene Symbol

RUFY1

UniProt

Additional RUFY1 Products

Product Documents for RUFY1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for RUFY1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.