RUNX1T1/ETO Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-59232

Novus Biologicals, part of Bio-Techne
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NBP2-59232
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Key Product Details

Species Reactivity

Human

Applications

Chromatin Immunoprecipitation (ChIP), Chromatin Immunoprecipitation Sequencing, ELISA

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all RUNX1T1/ETO Antibodies »

Product Specifications

Immunogen

The exact immunogen is propietary information.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RUNX1T1/ETO Antibody

Chromatin Immunoprecipitation: RUNX1T1/ETO Antibody [NBP2-59232] - ChIP assays were performed using SKNO-1 cells, the antibody against ETO and optimized primer pairs for qPCR. Sheared chromatin from 1.25 million cells and 4 uL of antibody were used per ChIP experiment. QPCR was performed using primers specific for the FUT7, NFE2, OGG1 and VEGF genes. Figure shows the occupancy, calculated as the ratio + control/background for which the H2B gene was used.
ELISA: RUNX1T1/ETO Antibody [NBP2-59232]

ELISA: RUNX1T1/ETO Antibody [NBP2-59232]

ELISA: RUNX1T1/ETO Antibody [NBP2-59232] - To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody directed against human ETO. The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:1,300.

Applications for RUNX1T1/ETO Antibody

Application
Recommended Usage

Chromatin Immunoprecipitation (ChIP)

4 uL/IP

ELISA

1:100

Formulation, Preparation, and Storage

Purification

Whole Antiserum

Formulation

Serum

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: RUNX1T1/ETO

RUNX1T1 / ETO is encoded by this gene is a putative zinc finger transcription factor and oncoprotein. In acute myeloid leukemia, especially in the M2 subtype, the t(8;21)(q22;q22) translocation is one of the most frequent karyotypic abnormalities. The translocation produces a chimeric gene made up of the 5'-region of the RUNX1 gene fused to the 3'-region of this gene. The chimeric protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation. Several transcript variants encoding multiple isoforms have been found for this gene.

Alternate Names

AML1T1protein CBFA2T1, CBFA2T1Cyclin-D-related protein, CDRMGC2796, core-binding factor, runt domain, alpha subunit 2; translocated to, 1; cyclinD-related, DKFZp564B213, Eight twenty one protein, ETOFLJ33145, MTG8acute myelogenous leukemia 1 translocation 1, cyclin-D related, Protein ETO, Protein MTG8, runt-related transcription factor 1; translocated to, 1 (cyclin D-related), Zinc finger MYND domain-containing protein 2, ZMYND2myeloid translocation gene on 8q22

Gene Symbol

RUNX1T1

Additional RUNX1T1/ETO Products

Product Documents for RUNX1T1/ETO Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for RUNX1T1/ETO Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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