RUNX1T1/ETO Antibody

Western Blot: RUNX1T1/ETO Antibody [NBP3-18685]

Western Blot: RUNX1T1/ETO Antibody [NBP3-18685] - Figure 2A: Construction of GST-fusion proteins containing different parts of RUNX1T1/ETO constructed and run on 15% polyacrylamide gel. Figure 2B: Western blot performed on seven GST-fusion proteins containing different fragments of RUNX1T1/ETO (1-7) with antibody against RUNX1T1/ETO , 1:1000 in TBS-Tween, 5% skimmed milk. MW marker (in kDa) is shown on the left; location of the protein of interest indicated on the right. Antibody raised against RUNX1T1/ETO recognizes ETO part (lane 2) of fusion protein. Figure 2C: Western blot performed on nuclear extracts from KAS-6/1 cells (human myeloma cell line) and SKNO-1 cells (human acute myeloblastic leukaemia) with antibody against RUNX1T1/ETO , diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Left, a MW marker is shown (in kDa). The location of RUNX1T1/ETO and a presumed splice variant (missing 106 C-terminal amino acids) are indicated on the right.

ELISA: RUNX1T1/ETO Antibody [NBP3-18685]

ELISA: RUNX1T1/ETO Antibody [NBP3-18685] - Figure 1. Determination of the antibody titer To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody directed against RUNX1T1/ETO , crude serum and flow through in antigen coated wells. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:5,250.