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RUNX3/CBFA3 Antibody (BLR122H)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-14706

Recombinant Monoclonal
Novus Biologicals, part of Bio-Techne
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NBP3-14706

Key Product Details

Species Reactivity

Validated:

Human

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit Clone # BLR122H

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Summary for RUNX3/CBFA3 Antibody (BLR122H)

Immunogen

between 1 and 50

Clonality

Monoclonal

Host

Rabbit

Scientific Data Images for RUNX3/CBFA3 Antibody (BLR122H)

Immunoprecipitation: RUNX3/CBFA3 Antibody (BLR122H) [NBP3-14706]

Immunoprecipitation: RUNX3/CBFA3 Antibody (BLR122H) [NBP3-14706]

Immunoprecipitation: RUNX3/CBFA3 Antibody [NBP3-14706] - Whole cell lysate (1.0 mgper IP reaction; 20% of IP loaded) from SK-MEL-28 cellsprepared using NETN lysis buffer. Antibodies: Rabbit antiRUNX3 recombinant monoclonal antibody used for IP at 20 ul/mg lysate. RUNX3was also immunoprecipitated by rabbit anti-RUNX3antibody. For blotting immunoprecipitatedRUNX3, was used at 1:1000.Chemiluminescence with an exposure time of 30 seconds.

Applications for RUNX3/CBFA3 Antibody (BLR122H)

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

>95%

Formulation

Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 2 - 8 C / 1 year from date of receipt

Background: RUNX3/CBFA3

RUNX3 binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, lck, IL-3 and GM-CSF promoters. RUNX3 consists of a heterodimer of an alpha and a beta subunit. The alpha subunit binds DNA as a monomer through the Runt domain. DNA-binding is increased by heterodimerization.

Long Name

Runt-related Transcription Factor 3

Alternate Names

AML2, CBFA3, PEBP2A3, PEBP2AC

Gene Symbol

RUNX3

Additional RUNX3/CBFA3 Products

Product Documents for RUNX3/CBFA3 Antibody (BLR122H)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for RUNX3/CBFA3 Antibody (BLR122H)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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