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Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # K12P3F11*C9

Format

BSA Free

Concentration

1 mg/ml

Product Summary for RUNX3/CBFA3 Antibody (K12P3F11*C9) - BSA Free

Immunogen

Peptide - QFDRSFPTL

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

44 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

The clone number is listed as K12P3F11*C9 in the scientific literature.

Scientific Data Images for RUNX3/CBFA3 Antibody (K12P3F11*C9) - BSA Free

Western Blot: RUNX3/CBFA3 Antibody (K12P3F11*C9)BSA Free [NBP2-50277]

Western Blot: RUNX3/CBFA3 Antibody (K12P3F11*C9)BSA Free [NBP2-50277]

Western Blot: RUNX3/CBFA3 Antibody (K12P3F11/C9) [NBP2-50277] - Detection of a 44 kDa band in a Jurkat cell lysate.

Applications for RUNX3/CBFA3 Antibody (K12P3F11*C9) - BSA Free

Application
Recommended Usage

ELISA

1:100 - 1:2000

Immunohistochemistry

1:10 - 1:500

Western Blot

1:100 - 1:2000
Application Notes
Positive control(s): WB - Jurkat cell lysates; IHC- formalin-fixed, paraffin-embedded multi tumour tissue microarray.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RUNX3/CBFA3

RUNX3 binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, lck, IL-3 and GM-CSF promoters. RUNX3 consists of a heterodimer of an alpha and a beta subunit. The alpha subunit binds DNA as a monomer through the Runt domain. DNA-binding is increased by heterodimerization.

Long Name

Runt-related Transcription Factor 3

Alternate Names

AML2, CBFA3, PEBP2A3, PEBP2AC

Gene Symbol

RUNX3

Additional RUNX3/CBFA3 Products

Product Documents for RUNX3/CBFA3 Antibody (K12P3F11*C9) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for RUNX3/CBFA3 Antibody (K12P3F11*C9) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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