RUVBL1 Antibody (3G4-1F8)

Novus Biologicals, part of Bio-Techne | Catalog # H00008607-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Proximity Ligation Assay, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 3G4-1F8

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all RUVBL1 Antibodies »

Product Specifications

Immunogen

RUVBL1 (AAH02993, 1 a.a. ~ 456 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MKIEEVKSTTKTQRIASHSHVKGLGLDESGLAKQAASGLVGQENAREACGVIVELIKSKKMAGRAVLLAGPPGTGKTALALAIAQELGSKVPFCPMVGSEVYSTEIKKTEVLMENFRRAIGLRIKETKEVYEGEVTELTPCETENPMGGYGKTISHVIIGLKTAKGTKQLKLDPSIFESLQKERVEAGDVIYIEANSGAVKRQGRCDTYATEFDLEAEEYVPLPKGDVHKKKEIIQDVTLHDLDVANARPQGGQDILSMMGQLMKPKKTEITDKLRGEINKVVNKYIDQGIAELVPGVLFVDEVHMLDIECFTYLHRALESSIAPIVIFASNRGNCVIRGTEDITSPHGIPLDLLDRVMIIRTMLYTPQEMKQIIKIRAQTEGINISEEALNHLGEIGTKTTLRYSVQLLTPANLLAKINGKDSIEKEHVEEISELFYDAKSSAKILADQQDKYMK

Specificity

RUVBL1 - RuvB-like 1 (E. coli)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for RUVBL1 Antibody (3G4-1F8)

Western Blot: RUVBL1 Antibody (3G4-1F8) [H00008607-M01]

Western Blot: RUVBL1 Antibody (3G4-1F8) [H00008607-M01]

Western Blot: RUVBL1 Antibody (3G4-1F8) [H00008607-M01] - RUVBL1 monoclonal antibody (M01), clone 3G4-1F8. Analysis of RUVBL1 expression in human pancreas.
Immunocytochemistry/ Immunofluorescence: RUVBL1 Antibody (3G4-1F8) [H00008607-M01]

Immunocytochemistry/ Immunofluorescence: RUVBL1 Antibody (3G4-1F8) [H00008607-M01]

Immunocytochemistry/Immunofluorescence: RUVBL1 Antibody (3G4-1F8) [H00008607-M01] - Analysis of monoclonal antibody to RUVBL1 on HeLa cell . Antibody concentration 10 ug/ml.
Western Blot: RUVBL1 Antibody (3G4-1F8) [H00008607-M01]

Western Blot: RUVBL1 Antibody (3G4-1F8) [H00008607-M01]

Western Blot: RUVBL1 Antibody (3G4-1F8) [H00008607-M01] - Analysis of RUVBL1 expression in transfected 293T cell line by RUVBL1 monoclonal antibody (M01), clone 3G4-1F8.Lane 1: RUVBL1 transfected lysate (Predicted MW: 50.2 KDa).Lane 2: Non-transfected lysate.
View 2 more images

Applications for RUVBL1 Antibody (3G4-1F8)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Proximity Ligation Assay

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against recombinant protein with GST tag on ELISA and WB. GST tag alone is used as a negative control. It has been use for IF.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: RUVBL1

Possesses single-stranded DNA-stimulated ATPase and ATP-dependent DNA helicase (3' to 5') activity. Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. The NuA4 complex ATPase and helicase activities seem to be, at least in part, contributed by the association of RUVBL1 and RUVBL2 with EP400. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. RUVBL1 plays an essential role in oncogenic transformation by MYC and also modulates transcriptional activation by the LEF1/TCF1-CTNNB1 complex

Alternate Names

49 kDa TATA box-binding protein-interacting protein, 54 kDa erythrocyte cytosolic protein, EC 3.6.1, EC 3.6.4.12,49 kDa TBP-interacting protein, ECP54, INO80 complex subunit H, INO80HTIP49A, NMP 238, NMP238ECP-54, Nuclear matrix protein 238, PONTIN, Pontin 52, Pontin52, RuvB (E coli homolog)-like 1, ruvB-like 1, RuvB-like 1 (E. coli), Rvb1, TATA binding protein interacting protein 49 kDa, TIH1, TIP49a, TIP49TAP54-alpha, TIP60-associated protein 54-alpha

Entrez Gene IDs

8607 (Human)

Gene Symbol

RUVBL1

OMIM

603449 (Human)

UniProt

AAH02993 (Human)

Additional RUVBL1 Products

Product Documents for RUVBL1 Antibody (3G4-1F8)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for RUVBL1 Antibody (3G4-1F8)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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