RUVBL1 Antibody

Immunohistochemistry-Paraffin: RUVBL1 Antibody [NBP2-20245]

Immunohistochemistry-Paraffin: RUVBL1 Antibody [NBP2-20245] - Human colon carcinoma, using RUVBL1antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Immunohistochemistry-Paraffin: RUVBL1 Antibody [NBP2-20245]

Immunohistochemistry-Paraffin: RUVBL1 Antibody [NBP2-20245] - Rat heart. RUVBL1 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Immunohistochemistry-Paraffin: RUVBL1 Antibody [NBP2-20245]

Immunohistochemistry-Paraffin: RUVBL1 Antibody [NBP2-20245] - Mouse colon. RUVBL1 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Immunohistochemistry-Paraffin: RUVBL1 Antibody [NBP2-20245]

Immunohistochemistry-Paraffin: RUVBL1 Antibody [NBP2-20245] - Rat testis. RUVBL1 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Immunoprecipitation: RUVBL1 Antibody [NBP2-20245]

Immunoprecipitation: RUVBL1 Antibody [NBP2-20245] - Immunoprecipitation of RUVBL protein from Jurkat whole cell extracts using 5 ug of RUVBL1 antibody. Western blot analysis was performed using RUVBL1 antibody. EasyBlot anti-Rabbit IgG was used as a secondary reagent.

Western Blot: RUVBL1 Antibody [NBP2-20245] -

RUVBL1/2 do not effect EBOV minigenome activity. (A) Schematic diagram of the EBOV minigenome system. The EBOV minigenome system consists of six plasmids: Four support plasmids encode replication complex components NP, L, VP35, & VP30. The EBOV minigenome plasmid encodes a firefly luciferase reporter gene flanked by the leader & trailer sequences of EBOV, & the plasmid that encodes Renilla luciferase is used for normalization. (B) Minigenome activity upon the knockdown of either RUVBL1, RUVBL2, or in combination. Below are protein levels confirmed by immunoblot. HeLa cells were transfected with 80 nM scrambled siRNA, 30 nM siRNA targeting RUVBL1, or 50 nM siRNA targeting RUVBL2. Twenty-four h after siRNA addition, the minigenome components were transfected. Forty-eight h later, minigenome reporter activity was measured. (C) Overexpression of FLAG-RUVBL1 & HA-RUVBL2 in the EBOV minigenome. HeLa cells were left untransfected, or transfected with vector control (VC), or increasing amounts of FLAG-RUVBL1 (125, 250, & 500 ng) or HA-RUVBL2 (125, 250, & 500 ng). Twenty-four h after exogenous transfection, the minigenome components were transfected. Forty-eight h later, minigenome reporter activity was measured. Data represent mean ± SEM from one representative experiment (n = 3) of at least three experiments (* p < 0.05). Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31018511), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: RUVBL1 Antibody [NBP2-20245] -

Immunocytochemistry/ Immunofluorescence: RUVBL1 Antibody [NBP2-20245] - Endogenous RUVBL1 & RUVBL2 colocalize with HA-NP. HeLa cells were transfected with vector control, HA-NP, or HA-VP35. Twenty-four h later, the cells were fixed & processed for immunofluorescence detection of endogenous RUVBL1 or RUVBL2 in the presence of vector control, HA-NP, or HA-VP35. Representative images of (A) endogenous RUVBL1 localization pattern with control vector (top panels), HA-NP (middle panels), or HA-VP35 (bottom panels) & (B) endogenous RUVBL2 localization pattern with control vector (top panels), HA-NP (middle panels), or HA-VP35 (bottom panels) are shown. HA-NP or HA-VP35 (green), RUVBL1/2 (red), & Hoechst 33342 nuclear stain (blue) were visualized by confocal microscopy. Scale bars = 20 µM. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31018511), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: RUVBL1 Antibody [NBP2-20245] -

Western Blot: RUVBL1 Antibody [NBP2-20245] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with RUVBL1 antibody (NBP2-20245) diluted at 1:1000.