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RUVBL2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-57593

Novus Biologicals, part of Bio-Techne
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NBP2-57593
NBP2-57593-25ul

Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Validated:

Human

Predicted:

Mouse (97%), Rat (97%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to the following amino acid sequence: ATVTDTTKVPEIRDVTRIERIGAHSHIRGLGLDDALEPRQASQGMVGQLAARRAAGVVLEMIREGKIAGR

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RUVBL2 Antibody

Immunohistochemistry-Paraffin: RUVBL2 Antibody [NBP2-57593]

Immunohistochemistry-Paraffin: RUVBL2 Antibody [NBP2-57593]

Immunohistochemistry-Paraffin: RUVBL2 Antibody [NBP2-57593] - Staining in human testis and liver tissues using anti-RUVBL2 antibody. Corresponding RUVBL2 RNA-seq data are presented for the same tissues.
Western Blot: RUVBL2 Antibody [NBP2-57593]

Western Blot: RUVBL2 Antibody [NBP2-57593]

Western Blot: RUVBL2 Antibody [NBP2-57593] - Analysis using Anti-RUVBL2 antibody NBP2-57593 (A) shows similar pattern to independent antibody NBP2-55231 (B).
Immunocytochemistry/ Immunofluorescence: RUVBL2 Antibody [NBP2-57593]

Immunocytochemistry/ Immunofluorescence: RUVBL2 Antibody [NBP2-57593]

Immunocytochemistry/Immunofluorescence: RUVBL2 Antibody [NBP2-57593] - Staining of human cell line A549 shows localization to nucleoplasm, cytosol & centrosome.

Applications for RUVBL2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:2500 - 1:5000

Immunohistochemistry-Paraffin

1:2500 - 1:5000

Western Blot

0.04-0.4 ug/ml
Application Notes
ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100. IHC-P, Retrieval method: HIER pH6.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RUVBL2

RUVBL2 encodes the second human homologue of the bacterial RuvB gene. Bacterial RuvB protein is a DNA helicase essential for homologous recombination and DNA double-strand break repair. Functional analysis showed that this gene product has both ATPase and DNA helicase activities. This gene is physically linked to the CGB/LHB gene cluster on chromosome 19q13.3, and is very close (55 nt) to the LHB gene, in the opposite orientation. [provided by RefSeq]

Alternate Names

48 kDa TATA box-binding protein-interacting protein, 51 kDa erythrocyte cytosolic protein, EC 3.6.1, EC 3.6.4.12,48 kDa TBP-interacting protein, ECP51TIP49B, erythrocyte cytosolic protein, 51-KD, INO80 complex subunit J, INO80JTAP54-beta, Repressing pontin 52, REPTIN, Reptin 52, Reptin52, RuvB (E coli homolog)-like 2, ruvB-like 2, RuvB-like 2 (E. coli), Rvb2, TBP-interacting protein, 48-KD, TIH2, TIP48ECP-51, TIP49b, TIP60-associated protein 54-beta

Gene Symbol

RUVBL2

Additional RUVBL2 Products

Product Documents for RUVBL2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for RUVBL2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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