Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all RWDD3 Antibodies »

Product Specifications

Immunogen

Antibody was raised against a 17 amino acid synthetic peptide from near the amino terminus of human RWDD3. The immunogen is located within the first 50 amino acids of RWDD3. Amino Acid Squence: CRPHEWEVLSRSETDGT

Reactivity Notes

Predicted species reactivity based on immunogen sequence: Bovine: (81%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RWDD3 Antibody - BSA Free

Western Blot: RWDD3 AntibodyBSA Free [NBP1-76309]

Western Blot: RWDD3 AntibodyBSA Free [NBP1-76309]

Western Blot: RWDD3 Antibody [NBP1-76309] - Mouse kidney tissue lysate with RWDD3 antibody at 0.5 ug/mL in (A) the absence and (B) the presence of blocking peptide.

Applications for RWDD3 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Western Blot

0.5 ug/ml

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RWDD3

RWDD3 (RSUME), a small RWD-containing protein, has a central role in sumoylation by enhancing SUMO conjugation in the regulatory network of immune-inflammatory signals. RWDD3 increases Ikappabeta sumoylation and stability. In addition, RWDD3 inhibits TNF-alpha-induced kappaB-LUC (Luciferase) reporter activity, showing the functional consequence of Ikappabeta increased stability. RSUME-enhanced sumoylation of Ikappabeta leads to the inhibition of NF-kappabeta activity on two well-known inflammatory genes, IL-8 and cyclooxygenase-2 (Cox-2) and therefore may also favor anti-inflammatory pathways. Expression of RWDD3 was induced under hypoxic conditions and it has a potential role during vascularization. Both BMP-4 and RWDD3 may be interesting targets for inhibiting steps involved in pituitary tumorigenesis.

Alternate Names

DKFZp566K023, FLJ51225, RSUME, RWD domain containing 3, RWD domain-containing protein 3, RWD-containing sumoylation enhancer

Gene Symbol

RWDD3

UniProt

NP_056300 (Human)

Additional RWDD3 Products

Product Documents for RWDD3 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for RWDD3 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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