RXR alpha/NR2B1 Antibody (JG99-38)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-75653

Recombinant Monoclonal Antibody.
Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # JG99-38

Concentration

1 mg/ml

View all RXR alpha/NR2B1 Antibodies »

Product Specifications

Immunogen

Recombinant protein corresponding to Human RXR alpha/NR2B1 aa 1-50 / 462. (SwissProt: P19793 Human; SwissProt: P28700 Mouse; SwissProt: Q05343 Rat)

Localization

Mitochondrion, Nucleus, Cytoplasm.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

51 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for RXR alpha/NR2B1 Antibody (JG99-38)

Western Blot: RXR alpha/NR2B1 Antibody (JG99-38) [NBP2-75653]

Western Blot: RXR alpha/NR2B1 Antibody (JG99-38) [NBP2-75653]

Western Blot: RXR alpha/NR2B1 Antibody (JG99-38) [NBP2-75653] - Western blot analysis of RXR alpha/NR2B1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: A549 cell lysate Lane 2: MCF-7 cell lysate
Immunocytochemistry/ Immunofluorescence: RXR alpha/NR2B1 Antibody (JG99-38) [NBP2-75653]

Immunocytochemistry/ Immunofluorescence: RXR alpha/NR2B1 Antibody (JG99-38) [NBP2-75653]

Immunocytochemistry/Immunofluorescence: RXR alpha/NR2B1 Antibody (JG99-38) [NBP2-75653] - Staining Retinoid X Receptor alpha in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: RXR alpha/NR2B1 Antibody (JG99-38) [NBP2-75653]

Immunohistochemistry-Paraffin: RXR alpha/NR2B1 Antibody (JG99-38) [NBP2-75653]

Immunohistochemistry-Paraffin: RXR alpha/NR2B1 Antibody (JG99-38) [NBP2-75653] - Analysis of paraffin-embedded rat liver tissue using anti-Retinoid X Receptor alpha antibody. Counter stained with hematoxylin.
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Applications for RXR alpha/NR2B1 Antibody (JG99-38)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50

Immunohistochemistry-Paraffin

1:50-1:400

Western Blot

1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

TBS (pH7.4), 0.05% BSA, 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RXR alpha/NR2B1

The steroid receptor superfamily acts through direct association with DNA sequences known as hormone response elements (HREs) and bind DNA as either homo- or heterodimers. The promiscuous mediator of heterodimerization, RXR, is the receptor for 9-cis reti

Long Name

Retinoid X Receptor, alpha

Alternate Names

NR2B1, RXRA

Gene Symbol

RXRA

Additional RXR alpha/NR2B1 Products

Product Documents for RXR alpha/NR2B1 Antibody (JG99-38)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for RXR alpha/NR2B1 Antibody (JG99-38)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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