RXR beta/NR2B2 Antibody (PCRP-RXRB-2B6) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-08445

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Protein Array, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # PCRP-RXRB-2B6

Format

Azide and BSA Free

Concentration

1 mg/ml

View all RXR beta/NR2B2 Antibodies »

Product Specifications

Immunogen

Recombinant full-length human RXR beta/NR2B2 protein (Uniprot: P28702)

Localization

Nucleus.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-07586).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for RXR beta/NR2B2 Antibody (PCRP-RXRB-2B6) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: RXR beta/NR2B2 Antibody (PCRP-RXRB-2B6) - Azide and BSA Free [NBP3-08445]

Immunocytochemistry/ Immunofluorescence: RXR beta/NR2B2 Antibody (PCRP-RXRB-2B6) - Azide and BSA Free [NBP3-08445]

Immunocytochemistry/Immunofluorescence: RXR beta/NR2B2 Antibody (PCRP-RXRB-2B6) [NBP3-08445] - Immunofluorescence Analysis of PFA-fixed K562 cells using RXR beta/NR2B2 Mouse Monoclonal Antibody (PCRP-RXRB-2B6) followed by goat anti- Mouse IgG-CF488 (green). CF640A phalloidin (red).
Immunohistochemistry-Paraffin: RXR beta/NR2B2 Antibody (PCRP-RXRB-2B6) - Azide and BSA Free [NBP3-08445]

Immunohistochemistry-Paraffin: RXR beta/NR2B2 Antibody (PCRP-RXRB-2B6) - Azide and BSA Free [NBP3-08445]

Immunohistochemistry-Paraffin: RXR beta/NR2B2 Antibody (PCRP-RXRB-2B6) [NBP3-08445] - Formalin-fixed, paraffin-embedded human lymph node stained with RXR beta/NR2B2 Mouse Monoclonal Antibody (PCRP-RXR beta/NR2B2-2B6).
Flow Cytometry: RXR beta/NR2B2 Antibody (PCRP-RXRB-2B6) - Azide and BSA Free [NBP3-08445]

Flow Cytometry: RXR beta/NR2B2 Antibody (PCRP-RXRB-2B6) - Azide and BSA Free [NBP3-08445]

Flow Cytometry: RXR beta/NR2B2 Antibody (PCRP-RXRB-2B6) [NBP3-08445] - Flow Cytometric Analysis of PFA-fixed HeLa cells. RXR beta/NR2B2 Mouse Monoclonal Antibody (PCRP-RXR beta/NR2B2-2B6) followed by goat anti- Mouse IgG-CF488 (blue); unstained cells (red).
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Applications for RXR beta/NR2B2 Antibody (PCRP-RXRB-2B6) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Western Blot

1-2 ug/ml
Application Notes
Immunohistochemistry Formalin-fixed: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: RXR beta/NR2B2

Retinoid X receptors (RXRs) are members of the steroid/thyroid hormone receptor superfamily of nuclear receptor proteins which exert their effects by binding to specific DNA response elements, thus regulating gene expression in target cells. The RXR subfamily consists of at least three similar genes, RXR alpha, RXR beta and RXR gamma, all of which control transcription of target genes mediated by retinoids. RXR beta controls expression of many genes that respond to hormones and vitamins, including thyroid hormone, estrogen, retinoids and vitamin D. RXR beta controls a wide array of genes because of its ability to heterodimerize with other hormone receptors including the thyroid hormone receptor (THR), vitamin D receptor (VDR) and retinoic acid receptor (RAR).

Long Name

Retinoid X Receptor, beta

Alternate Names

H-2RIIBP, NR2B2, RXRB

Gene Symbol

RXRB

Additional RXR beta/NR2B2 Products

Product Documents for RXR beta/NR2B2 Antibody (PCRP-RXRB-2B6) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for RXR beta/NR2B2 Antibody (PCRP-RXRB-2B6) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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