S-Tag Epitope Tag Antibody (247)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-43726
Key Product Details
Species Reactivity
Validated:
Epitope Tag
Applications
Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 247
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Summary for S-Tag Epitope Tag Antibody (247)
Immunogen
The immunogen used to generate this antibody corresponds to S-Tag Epitope Tag
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for S-Tag Epitope Tag Antibody (247)
![Western Blot: S-Tag Epitope Tag Antibody (247) [NBP2-43726]](https://resources.bio-techne.com/images/products/S-Tag-Epitope-Tag-Antibody-247-Western-Blot-NBP2-43726-img0002.jpg "Western Blot: S-Tag Epitope Tag Antibody (247) [NBP2-43726]")
Western Blot: S-Tag Epitope Tag Antibody (247) [NBP2-43726]
Western Blot: S-Tag Epitope Tag Antibody (247) [NBP2-43726] - Analysis of A. 30 ug 293T whole cell lysate/eztract B. 30 ug whole cell lysate/extract of S-tagged protein expressing 293T cells 12 % SDS-PAGE S Tag antibody dilution: 1:5000.![Western Blot: S-Tag Epitope Tag Antibody (247) [NBP2-43726]](https://resources.bio-techne.com/images/products/S-Tag-Epitope-Tag-Antibody-247-Western-Blot-NBP2-43726-img0001.jpg "Western Blot: S-Tag Epitope Tag Antibody (247) [NBP2-43726]")
Western Blot: S-Tag Epitope Tag Antibody (247) [NBP2-43726]
Western Blot: S-Tag Epitope Tag Antibody (247) [NBP2-43726] - Analysis of 400 ug cell lysate/extract of S-fused gene transfected 293T cells A. 30 ug lysate/extract of transfected 293T cell B. Control with 2 ug of preimmune mouse IgG C. Immunoprecipitation of S-fused protein by 2 ug of S tag antibody 12% SDS-PAGE The immunoprecipitated S-fused protein was detected by S tag antibody diluted at 1:1000. EasyBlot anti-mouse IgG was used as a secondary reagent.Applications for S-Tag Epitope Tag Antibody (247)
Application
Recommended Usage
Immunoprecipitation
1:500-1:1000
Western Blot
1:1000-1:10000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: S-Tag Epitope Tag
Alternate Names
KETAAAKFERQHMDS, S 7 Epitope tag
Additional S-Tag Epitope Tag Products
Product Documents for S-Tag Epitope Tag Antibody (247)
Product Specific Notices for S-Tag Epitope Tag Antibody (247)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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